Application
Fig1: Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution. Positive control： Lane1: Mouse liver tissue Lane2: Rat liver tissue Lane3: HepG2Application
Fig2: ICC staining GRP78 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GRP78 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining GRP78 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRP78 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP78 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-GRP78 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GRP78 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP78 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Jurkat cells with GRP78 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Product Name | GRP78 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
| Product description | Plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate (By similarity). GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. |
| Immunogen | Peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 78 kDa glucose regulated protein antibody 78 kDa glucose-regulated protein antibody AL022860 antibody AU019543 antibody BIP antibody D2Wsu141e antibody D2Wsu17e antibody Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody Epididymis secretory sperm binding protein Li 89n antibody FLJ26106 antibody Glucose Regulated Protein 78kDa antibody GRP 78 antibody GRP-78 antibody GRP78 antibody GRP78_HUMAN antibody Heat shock 70 kDa protein 5 antibody Heat Shock 70kDa Protein 5 antibody Heat shock protein family A (Hsp70) member 5 antibody HEL S 89n antibody Hsce70 antibody HSPA 5 antibody HSPA5 antibody Immunoglobulin Heavy Chain Binding Protein antibody Immunoglobulin heavy chain-binding protein antibody mBiP antibody MIF2 antibody Sez7 antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Endoplasmic reticulum. Cytoplasm. |
Application
Fig1: Western blot analysis of GRP78 on different lysates using anti-GRP78 antibody at 1/500 dilution. Positive control： Lane1: Mouse liver tissue Lane2: Rat liver tissue Lane3: HepG2
Application
Fig2: ICC staining GRP78 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GRP78 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining GRP78 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GRP78 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Jurkat cells with GRP78 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse liver tissue lysate, rat liver tissue lysate, HepG2, A431, HUVEC, rat brain tissue, human liver tissue, human placenta tissue, human stomach cancer tissue, mouse cerebellum tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
