Application
Fig1: ICC staining CD21 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining CD21 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of LOVO cells with CD21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD21 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | C3DR antibody CD 21 antibody CD21 antibody Complement C3d receptor 2 antibody Complement C3d receptor antibody Complement component (3d/Epstein Barr virus) receptor 2 antibody Complement receptor type 2 antibody CR antibody Cr2 antibody CR2_HUMAN antibody CVID7 antibody EBV receptor antibody EBV-R antibody Epstein Barr virus receptor antibody Epstein-Barr virus receptor antibody EVBR antibody SLEB9 antibody |
| Product description | CD21 (also known as complement receptor (CR) type 2) is a type I integral membrane glycoprotein that serves as a receptor for the C3d complement fragment and for the Epstein-Barr virus. It plays a role in B cell activation and proliferation and undergoes phosphorylation after B cell activation with phorbol esters. CD21 is expressed on mature B cells, follicular dendritic cells, pharyngeal and cervical epithelial cells and a subset of thymocytes. The adaptive immune response is tightly regulated to limit responding cells in an antigen-specific manner. On B cells, co-receptors CD21/CD19 modulate the strength of B cell Ag receptor (BCR) signals, thereby influencing cell fate. CD21 is normally expressed during the immature and mature stages of B cell development. In association with CD19, CR21 plays an important role in enhancing mature B cell responses to foreign antigens. |
| Immunogen | Peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHCFC |
| IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | C3DR antibody CD 21 antibody CD21 antibody Complement C3d receptor 2 antibody Complement C3d receptor antibody Complement component (3d/Epstein Barr virus) receptor 2 antibody Complement receptor type 2 antibody CR antibody Cr2 antibody CR2_HUMAN antibody CVID7 antibody EBV receptor antibody EBV-R antibody Epstein Barr virus receptor antibody Epstein-Barr virus receptor antibody EVBR antibody SLEB9 antibody |
|---|---|
| Cellular Localization | Membrane. |
| SwissProt ID | P20023 |
|---|
Application
Fig1: ICC staining CD21 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining CD21 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of LOVO cells with CD21 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | LOVO, SH-SY5Y, human tonsil tissue, human colon cancer tissue. |
|---|---|
| Application Notes | IHC:1:50-1:200 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
