Application
Fig1: Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution. Positive control： Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissueApplication
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Cullin-3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CUL 3 antibody Cul-3 antibody cul3 antibody CUL3_HUMAN antibody Cullin-3 antibody Cullin3 antibody KIAA0617 antibody PHA2E antibody |
| Product description | Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1. |
| Immunogen | Peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | CUL 3 antibody
Cul-3 antibody cul3 antibody CUL3_HUMAN antibody Cullin-3 antibody Cullin3 antibody KIAA0617 antibody PHA2E antibody |
|---|---|
| Molecular Weight(MW) | 89 kDa |
| Cellular Localization | Golgi apparatus. Nucleus. |
Application
Fig1: Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution. Positive control： Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissue
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
