Application
Fig1: Western blot analysis of NLRP3 on different lysates using anti-NLRP3 antibody at 1/1,000 dilution. Positive control： Lane1: Human lung Lane2: Mouse thymus Lane3: THP-1Application
Fig2: ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | NLRP3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGTAVPRL antibody AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like antibody Angiotensin/vasopressin receptor AII/AVP-like antibody C1orf7 antibody Caterpiller protein 1.1 antibody CIAS 1 antibody CIAS1 antibody CLR1.1 antibody Cold autoinflammatory syndrome 1 antibody Cold autoinflammatory syndrome 1 protein antibody Cryopyrin antibody Familial cold autoinflammatory syndrome antibody FCAS antibody FCU antibody LRR and PYD domains-containing protein 3 antibody Muckle-Wells syndrome antibody MWS antibody NACHT antibody NACHT LRR and PYD containing protein 3 antibody NALP 3 antibody NALP3 antibody NALP3_HUMAN antibody NLR family pyrin domain containing 3 antibody NLRP3 antibody PYPAF 1 antibody PYPAF1 antibody PYRIN containing APAF1 like protein 1 antibody PYRIN-containing APAF1-like protein 1 antibody |
| Product description | Cryopyrin interacts selectively with apoptosis associated specklike protein containing a CARD domain (ASC). This complex may function as an upstream activator of NFkB signaling and caspase-1 activation. The complex also inhibits TNFa induced activation and nuclear translocation of RelA/NFkB p65. Mutations in Cryopyrin and Pyrin proteins are responsible for several autoinflammatory disorders in humans, including familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS) and chronic infantile neurologic cutaneous and articular syndrome (CINCA). |
| Immunogen | Peptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:2,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | AGTAVPRL antibody AII/AVP antibody Angiotensin/vasopressin receptor AII/AVP like antibody Angiotensin/vasopressin receptor AII/AVP-like antibody C1orf7 antibody Caterpiller protein 1.1 antibody CIAS 1 antibody CIAS1 antibody CLR1.1 antibody Cold autoinflammatory syndrome 1 antibody Cold autoinflammatory syndrome 1 protein antibody Cryopyrin antibody Familial cold autoinflammatory syndrome antibody FCAS antibody FCU antibody LRR and PYD domains-containing protein 3 antibody Muckle-Wells syndrome antibody MWS antibody NACHT antibody NACHT LRR and PYD containing protein 3 antibody NALP 3 antibody NALP3 antibody NALP3_HUMAN antibody NLR family pyrin domain containing 3 antibody NLRP3 antibody PYPAF 1 antibody PYPAF1 antibody PYRIN containing APAF1 like protein 1 antibody PYRIN-containing APAF1-like protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Secreted. |
Application
Fig1: Western blot analysis of NLRP3 on different lysates using anti-NLRP3 antibody at 1/1,000 dilution. Positive control： Lane1: Human lung Lane2: Mouse thymus Lane3: THP-1
Application
Fig2: ICC staining NLRP3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NLRP3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NLRP3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-NLRP3 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of Jurkat cells with NLRP3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Human lung tissue lysate, mouse thymus tissue lysate, THP-1, A431, HepG2, LOVO, human liver cancer tissue, human colon cancer tissue, human placenta tissue, mouse brain tissue, mouse kidney tissue, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
