Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ATP citrate lyase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACL antibody Acly antibody ACLY_HUMAN antibody ATP citrate (pro-S) lyase antibody ATP citrate lyase antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody ATPcitrate synthase antibody ATPCL antibody Citrate cleavage enzyme antibody CLATP antibody OTTHUMP00000164773 antibody |
| Product description | ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer of apparently identical subunits. The product, acetyl-CoA, in animals serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. It is activated by insulin. In plants, ATP citrate lyase generates the acetyl-CoA for cytosolically-synthesized metabolites. (Acetyl-CoA is not transported across subcellular membranes of plants.) These include: elongated fatty acids (used in seed oils, membrane phospholipids, the ceramide moiety of sphingolipids, cuticle, cutin, and suberin); flavonoids; malonic acid; acetylated phenolics, alkaloids, isoprenoids, anthocyanins, and sugars; and, mevalonate-derived isoprenoids (e.g., sesquiterpenes, sterols, brassinosteroids); malonyl and acyl-derivatives (d-amino acids, malonylated flavonoids, acylated, prenylated and malonated proteins). De novo fatty acid biosynthesis in plants is plastidic, thus ATP citrate lyase is not important for this pathway. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ACL antibody Acly antibody ACLY_HUMAN antibody ATP citrate (pro-S) lyase antibody ATP citrate lyase antibody ATP citrate synthase antibody ATP-citrate (pro-S-)-lyase antibody ATP-citrate synthase antibody ATPcitrate synthase antibody ATPCL antibody Citrate cleavage enzyme antibody CLATP antibody OTTHUMP00000164773 antibody |
|---|---|
| Molecular Weight(MW) | 122 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig8: Flow cytometric analysis of A549 cells with ATP citrate lyase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | NIH-3T3, A549, SH-SY5Y, mouse pancreas tissue lysate, rat brain tissue, human colon cancer tissue, human pancreas tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
