Application
Fig1: Western blot analysis of USP11 on 293T cell using anti-USP11 antibody at 1/1,000 dilution.Application
Fig2: ICC staining USP11 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining USP11 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining USP11 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of Jurkat cells with USP11 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | USP11 [JM33-62] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deubiquitinating enzyme 11 antibody Ubiquitin carboxyl-terminal hydrolase 11 antibody Ubiquitin carboxyl-terminal hydrolase X linked antibody ubiquitin specific peptidase 11 antibody Ubiquitin specific protease 11 antibody Ubiquitin thiolesterase 11 antibody Ubiquitin-specific-processing protease 11 antibody UBP11_HUMAN antibody UHX1 antibody USP11 antibody |
| Product description | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP11 (ubiquitin specific peptidase 11), also known as UHX1, is a 920 amino acid deubiquitinating enzyme that participates in the Ub pathway. Localized to the nucleus, USP11 associates with both Ran BP-M (Ran binding protein M) and with the tumor suppressor BRCA2. Through these associations, USP11 functions to either inhibit ubiquitination of these proteins or to remove ubiquitin residues that have already been attached to these proteins. USP11 is implicated in several X-linked retinal diseases and, due to its ability to deubiquitinate BRCA2, may play a role in tumor suppression. |
| Immunogen | recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIPFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Deubiquitinating enzyme 11 antibody Ubiquitin carboxyl-terminal hydrolase 11 antibody Ubiquitin carboxyl-terminal hydrolase X linked antibody ubiquitin specific peptidase 11 antibody Ubiquitin specific protease 11 antibody Ubiquitin thiolesterase 11 antibody Ubiquitin-specific-processing protease 11 antibody UBP11_HUMAN antibody UHX1 antibody USP11 antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Nucleus. Cytoplasm. Predominantly nuclear. |
Application
Fig1: Western blot analysis of USP11 on 293T cell using anti-USP11 antibody at 1/1,000 dilution.
Application
Fig2: ICC staining USP11 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining USP11 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining USP11 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of Jurkat cells with USP11 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | 293T, A549, HepG2, SH-SY5Y, Hela. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
