Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | FBXW7 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AGO antibody Archipelago antibody Archipelago homolog antibody Archipelago, Drosophila, homolog of antibody CDC4 antibody CDC4, S.cerevisiae, homolog of antibody DKFZp686F23254 antibody F box and WD 40 domain protein 7 (archipelago homolog, Drosophila) antibody F box and WD 40 domain protein 7 antibody F box and WD repeat domain containing 7 antibody F box protein FBW7 antibody F box protein FBX30 antibody F box protein SEL 10 antibody F box protein SEL10 antibody F-box and WD repeat domain containing 7, E3 ubiquitin protein ligase antibody F-box and WD-40 domain-containing protein 7 antibody F-box protein FBX30 antibody F-box/WD repeat-containing protein 7 antibody FBW6 antibody FBW7 antibody FBX30 antibody FBXO30 antibody FBXW6 antibody FBXW7 antibody FBXW7_HUMAN antibody FLJ16457 antibody hAgo antibody hCdc4 antibody Homolog of C elegans sel 10 antibody Homolog of C.elegans sel10 antibody SEL 10 antibody SEL-10 antibody SEL10 antibody |
| Product description | Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds phosphorylated sites/phosphodegrons within target proteins and thereafter bring them to the SCF complex for ubiquitination. Identified substrates include cyclin-E (CCNE1 or CCNE2), JUN, MYC, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. Acts as a negative regulator of JNK signaling by binding to phosphorylated JUN and promoting its ubiquitination and subsequent degradation |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFCIHC |
| WB:1:500-1:1,000 ICC:1:500-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AGO antibody
Archipelago antibody
Archipelago homolog antibody
Archipelago Drosophila homolog of antibody CDC4 antibody CDC4 S.cerevisiae homolog of antibody DKFZp686F23254 antibody F box and WD 40 domain protein 7 (archipelago homolog Drosophila) antibody F box and WD 40 domain protein 7 antibody F box and WD repeat domain containing 7 antibody F box protein FBW7 antibody F box protein FBX30 antibody F box protein SEL 10 antibody F box protein SEL10 antibody F-box and WD repeat domain containing 7 E3 ubiquitin protein ligase antibody F-box and WD-40 domain-containing protein 7 antibody F-box protein FBX30 antibody F-box/WD repeat-containing protein 7 antibody FBW6 antibody FBW7 antibody FBX30 antibody FBXO30 antibody FBXW6 antibody FBXW7 antibody FBXW7_HUMAN antibody FLJ16457 antibody hAgo antibody hCdc4 antibody Homolog of C elegans sel 10 antibody Homolog of C.elegans sel10 antibody SEL 10 antibody SEL-10 antibody SEL10 antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Application
Fig4: Flow cytometric analysis of SH-SY5Y cells with FBXW7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse spleen tissue lysate, rat lung tissue lysate, HepG2, Hela, HUVEC, SH-SY5Y. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:500-1:2,000 FC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
