Application
Fig1: Western blot analysis of TrkA on SHSY5Y (1) and SHG-44 (2) cell lysates using anti-TrkA antibody at 1/200 dilution.Application
Fig2: ICC staining TrkA in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of SHSY5Y cells with TrkA 1/2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Product Name | TrkA |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | gp140trk antibody High affinity nerve growth factor receptor antibody High affinity nerve growth factor receptor precursor antibody MTC antibody Neurotrophic tyrosine kinase receptor type 1 antibody NTRK1 antibody NTRK1_HUMAN antibody Oncogene TRK antibody p14-TrkA antibody p140 TrkA antibody p140-TrkA antibody Slow nerve growth antibody Trk A antibody TRK antibody Trk-A antibody TRK1 antibody TRK1-transforming tyrosine kinase protein antibody Tropomyosin-related kinase A antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody |
| Product description | The Trk proto-oncogene encodes a tyrosine protein kinase, Trk A, also designated Trk gp140, that serves as a receptor for certain neurotrophic factors including nerve growth factor (NGF) and neurotrophin-3 (NT-3). Trk B is a tyrosine kinase gene highly related to Trk A. Trk B expression is confined to tissues within the central and peripheral nervous systems. The brain-derived neurotrophic factor (BDNF) and NT-3, but not NGF, can induce rapid phosphorylation on Tyrosine of Trk B gp145, one of the receptors encoded by Trk B, although BDNF elicits a response at least two orders of magnitude greater than NT-3. Thus it appears that Trk B gp145 may represent a neurotrophic receptor for BDNF and NT-3. The third member of the Trk family of tyrosine kinases, Trk C, encodes a protein designated Trk C gp145 that is preferentially expressed in brain tissue, is equally related to Trk A and Trk B and is a functional receptor for NT-3. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:100 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | gp140trk antibody High affinity nerve growth factor receptor antibody High affinity nerve growth factor receptor precursor antibody MTC antibody Neurotrophic tyrosine kinase receptor type 1 antibody NTRK1 antibody NTRK1_HUMAN antibody Oncogene TRK antibody p14-TrkA antibody p140 TrkA antibody p140-TrkA antibody Slow nerve growth antibody Trk A antibody TRK antibody Trk-A antibody TRK1 antibody TRK1-transforming tyrosine kinase protein antibody Tropomyosin-related kinase A antibody Tyrosine kinase receptor A antibody Tyrosine kinase receptor antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 87 kDa |
| Cellular Localization | Cell membrane. Endosome. |
Application
Fig1: Western blot analysis of TrkA on SHSY5Y (1) and SHG-44 (2) cell lysates using anti-TrkA antibody at 1/200 dilution.
Application
Fig2: ICC staining TrkA in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TrkA antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of SHSY5Y cells with TrkA 1/2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.| Positive Control | SHSY5Y, SHG-44, PC-12, Human liver cancer tissue, human stomach cancer tissue, mouse brain tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:100 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
