Application
Fig1: ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-iNOS antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-iNOS antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-iNOS antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | iNOS |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HEP-NOS antibody Hepatocyte NOS antibody HEPNOS antibody inducible antibody Inducible nitric oxide synthase antibody Inducible NO synthase antibody Inducible NOS antibody iNOS antibody MAC NOS antibody Macrophage NOS antibody Nitric oxide synthase 2 inducible antibody Nitric oxide synthase 2 inducible macrophage antibody nitric oxide synthase 2A (inducible, hepatocytes) antibody Nitric oxide synthase antibody Nitric oxide synthase inducible antibody nitric oxide synthase, macrophage antibody NOS 2 antibody NOS antibody Nos II antibody NOS type II antibody nos2 antibody NOS2_HUMAN antibody NOS2A antibody NOS2A, Inducible, Hepatocyte antibody Peptidyl-cysteine S-nitrosylase NOS2 antibody |
| Product description | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICCIHCFCWB |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | HEP-NOS antibody
Hepatocyte NOS antibody
HEPNOS antibody
inducible antibody
Inducible nitric oxide synthase antibody
Inducible NO synthase antibody
Inducible NOS antibody
iNOS antibody
MAC NOS antibody
Macrophage NOS antibody
Nitric oxide synthase 2 inducible antibody
Nitric oxide synthase 2 inducible macrophage antibody
nitric oxide synthase 2A (inducible hepatocytes) antibody Nitric oxide synthase antibody Nitric oxide synthase inducible antibody nitric oxide synthase macrophage antibody NOS 2 antibody NOS antibody Nos II antibody NOS type II antibody nos2 antibody NOS2_HUMAN antibody NOS2A antibody NOS2A Inducible Hepatocyte antibody Peptidyl-cysteine S-nitrosylase NOS2 antibody |
|---|---|
| Molecular Weight(MW) | 131 kDa |
| Cellular Localization | Cytoskeleton. Nucleus. Cytosol. |
Application
Fig1: ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | A549, LOVO, human tonsil tissue, human spleen tissue, mouse small intestine tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
