Application
Fig1: Western blot analysis of PKC beta 2 on different lysates using anti-PKC beta 2 antibody at 1/500 dilution. Positive control： Lane1: Rat spleen tissue Lane2: JurkatApplication
Fig2: ICC staining PKC beta 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PKC beta 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.| Product Name | PKC beta |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPCB_HUMAN antibody PKC beta antibody PKC-B antibody PKC-beta antibody PKCB antibody Prkcb antibody PRKCB I antibody PRKCB1 antibody PRKCB2 antibody Protein kinase C beta antibody Protein kinase C beta type antibody protein kinase C, beta 1 polypeptide antibody protein kinase C, beta-1 antibody |
| Product description | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | KPCB_HUMAN antibody
PKC beta antibody
PKC-B antibody
PKC-beta antibody
PKCB antibody
Prkcb antibody
PRKCB I antibody
PRKCB1 antibody
PRKCB2 antibody
Protein kinase C beta antibody
Protein kinase C beta type antibody
protein kinase C beta 1 polypeptide antibody protein kinase C beta-1 antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of PKC beta 2 on different lysates using anti-PKC beta 2 antibody at 1/500 dilution. Positive control： Lane1: Rat spleen tissue Lane2: Jurkat
Application
Fig2: ICC staining PKC beta 2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PKC beta 2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.| Positive Control | Rat spleen tissue lysate, Hela, HepG2, human tonsil tissue, human spleen tissue, mouse spleen tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
