Application
Fig1: Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution.Application
Fig2: ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Flow cytometric analysis of Jurkat cells with SATB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | SATB1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA binding protein SATB1 antibody DNA-binding protein SATB1 antibody SATB homeobox 1 antibody SATB1 antibody SATB1_HUMAN antibody Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody Special AT rich sequence binding protein 1 antibody Special AT-rich sequence-binding protein 1 antibody |
| Product description | The homeoproteins CCAAT displacement protein (CDP) and special AT-rich sequence binding protein 1 (SATB1) are transcriptional repressors of many cellular genes, and they participate in cell development and cell type differentiation. SATB1 is expressed primarily in thymocytes, and, like CDP, it also contains a distinct homeobox DNA-binding domain that is essential for DNA binding. SATB1 and CDP interact through these homeodomains and synergistically function as mediators of gene expression. SATB1 contains an additional domain that has a higher affinity for DNA and specifically facilitates the direct association between SATB1 and the nuclear matrix attachment regions (MARs) of DNA. MARs are specific DNA sequences that bind to the nuclear matrix and form the base of chromosomal loops that organize the chromosomes and regulate DNA transcription and replication within the nucleus. The association of SATB1 with the core unwinding element within the base-unpairing region of MARs requires both the MAR and homeobox binding domains of SATB1. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:500 ICC:1:100 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | DNA binding protein SATB1 antibody DNA-binding protein SATB1 antibody SATB homeobox 1 antibody SATB1 antibody SATB1_HUMAN antibody Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody Special AT rich sequence binding protein 1 antibody Special AT-rich sequence-binding protein 1 antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 86 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of SATB1 on mouse thymus tissue lysate using anti-SATB1 antibody at 1/500 dilution.
Application
Fig2: ICC staining SATB1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Flow cytometric analysis of Jurkat cells with SATB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | Mouse thymus tissue lysate,SH-SY-5Y, Jurkat. |
|---|---|
| Application Notes | WB:1:500 ICC:1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
