Application
Fig1: Western blot analysis of CXCR4 on different cell lysates using anti-CXCR4 antibody at 1/500 dilution. Positive control： Lane1: 293 Lane2: K562Application
Fig2: ICC staining CXCR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining CXCR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of THP-1 cells with CXCR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CXCR4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The C-X-C or a chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 (SDF-1). SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemo-attractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB and CXCR-4 (also known as LESTR or fusin). CXCR-4 is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. CXCR-4 has been identified as the major co-receptor for T-tropic HIV-1 and SDF-1 has been shown to inhibit HIV-1 infection. |
| Immunogen | Peptide |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FCICCIHCWB |
| WB:1:500-1:1,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ |
| Alternative Names | C-X-C chemokine receptor type 4 antibody CD184 antibody CD184 antigen antibody Chemokine (C X C motif) receptor 4 antibody Chemokine CXC Motif Receptor 4 antibody CXC-R4 antibody CXCR-4 antibody CXCR4 antibody CXCR4_HUMAN antibody D2S201E antibody FB22 antibody Fusin antibody HM89 antibody HSY3RR antibody LAP 3 antibody LAP3 antibody LCR1 antibody LESTR antibody Leukocyte derived seven transmembrane domain receptor antibody Leukocyte-derived seven transmembrane domain receptor antibody Lipopolysaccharide associated protein 3 antibody Neuropeptide Y receptor Y3 antibody NPY3R antibody NPYR antibody NPYRL antibody NPYY3 antibody NPYY3R antibody Probable G protein coupled receptor lcr1 homolog antibody SDF 1 receptor antibody SDF-1 receptor antibody SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody Stromal cell derived factor 1 receptor antibody Stromal cell-derived factor 1 receptor antibody WHIM antibody WHIMS antibody |
|---|---|
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cell junction. Plasma membrane. Endosome. Lysosome. |
Application
Fig1: Western blot analysis of CXCR4 on different cell lysates using anti-CXCR4 antibody at 1/500 dilution. Positive control： Lane1: 293 Lane2: K562
Application
Fig2: ICC staining CXCR4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining CXCR4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-CXCR4 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of THP-1 cells with CXCR4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293, K562, Hela, HUVEC, rat lung tissue, human tonsil tissue, human liver tissue, THP-1. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
