Application
Fig1: Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.Application
Fig2: ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Noggin antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of PC-3M cells with Noggin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Product Name | Noggin |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nog antibody NOGG_HUMAN antibody Noggin antibody SYM 1 antibody SYM1 antibody Symphalangism 1 (proximal) antibody Synostoses (multiple) syndrome 1 antibody SYNS 1 antibody SYNS1 antibody |
| Product description | Genetic differentiation of the vertebrate somite necessitates a balance of inductive signals and antagonists. Noggin is a secreted protein that binds and inactivates members of the transforming growth factor-beta (TGFβ) superfamily of signaling proteins, such as bone morphogenetic proteins-2, 4, 7 (BMP2, BMP4, BMP7). Inhibition of BMP signaling by axially secreted Noggin mediates normal vertebrate skeletogenesis and patterning of the neural tube and somite. Spatially, Noggin may effectively antagonize BMP activity by efficiently diffusing through extracellular matrices, thereby creating morpho-genic gradients. Mice embryos that are homozygous null for Noggin, a lethal genotype, display stubby, continuous limbs with lack of joints in the paws and an array of other developmental defects. |
| Immunogen | Recombinant protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | Nog antibody NOGG_HUMAN antibody Noggin antibody SYM 1 antibody SYM1 antibody Symphalangism 1 (proximal) antibody Synostoses (multiple) syndrome 1 antibody SYNS 1 antibody SYNS1 antibody |
|---|---|
| Molecular Weight(MW) | 26 kDa |
| Cellular Localization | Secreted. |
Application
Fig1: Western blot analysis of Noggin on Recombinant protein using anti-Noggin antibody at 1/500 dilution.
Application
Fig2: ICC staining Noggin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Noggin antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Noggin antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of PC-3M cells with Noggin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).| Positive Control | Noggin Recombinant protein, PC-3M, rat liver tissue, human liver tissue, human placenta tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
