Application
Fig1: Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: Mouse liver Lane 4: Rat liver Lane 5: Rat spleenApplication
Fig2: ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin.Application
Fig9: Western blot analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution.| Product Name | Aldolase [JM54-19] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALDA antibody Aldo1 antibody ALDOA antibody ALDOA_HUMAN antibody Aldolase 1 antibody Aldolase A antibody Aldolase A fructose bisphosphatase antibody Aldolase A fructose bisphosphate antibody Aldolase, fructose-bisphosphate A antibody Epididymis secretory sperm binding protein Li 87p antibody FRUCTOALDOLASE A antibody Fructose 1 6 bisphosphate triosephosphate lyase antibody Fructose bisphosphate aldolase A antibody Fructose bisphosphate aldolase antibody FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A antibody Fructose-bisphosphate aldolase A antibody Fructose-bisphosphate aldolase A Muscle-type antibody GSD12 antibody HEL S 87p antibody Lung cancer antigen NY LU 1 antibody Lung cancer antigen NY-LU-1 antibody MGC10942 antibody MGC17716 antibody MGC17767 antibody Muscle type aldolase antibody Muscle-type aldolase antibody RNALDOG5 antibody |
| Product description | Fructose 1,6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1,6-bisphosphate. Fructose 1,6-bisphosphate aldolase exists as three forms: the muscle-specific Aldolase A; the liver-specific Aldolase B; and the brain-specific Aldolase C. Aldolase A, B and C arose from a common ancestral gene from which Aldo-lase B first diverged. Aldolase A is one of the most highly-conserved enzymes known, with only about 2% of the residues changing per 100 million years. Aldolase B is regulated by the hormones insulin and glucagon, and has been implicated in hereditary fructose intolerance disease. Aldolase C is a polypeptide that is exclusively expressed in Purkinje cells. Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHC |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ALDA antibody
Aldo1 antibody
ALDOA antibody
ALDOA_HUMAN antibody
Aldolase 1 antibody
Aldolase A antibody
Aldolase A fructose bisphosphatase antibody
Aldolase A fructose bisphosphate antibody
Aldolase fructose-bisphosphate A antibody Epididymis secretory sperm binding protein Li 87p antibody FRUCTOALDOLASE A antibody Fructose 1 6 bisphosphate triosephosphate lyase antibody Fructose bisphosphate aldolase A antibody Fructose bisphosphate aldolase antibody FRUCTOSE-1 6-BISPHOSPHATE ALDOLASE A antibody Fructose-bisphosphate aldolase A antibody Fructose-bisphosphate aldolase A Muscle-type antibody GSD12 antibody HEL S 87p antibody Lung cancer antigen NY LU 1 antibody Lung cancer antigen NY-LU-1 antibody MGC10942 antibody MGC17716 antibody MGC17767 antibody Muscle type aldolase antibody Muscle-type aldolase antibody RNALDOG5 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Aldolase on different lysates using anti-Aldolase antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: Mouse liver Lane 4: Rat liver Lane 5: Rat spleen
Application
Fig2: ICC staining Aldolase in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Aldolase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Aldolase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Aldolase antibody. Counter stained with hematoxylin.
Application
Fig9: Western blot analysis of Aldolase on Zebrafish tissue lysates using anti-Aldolase antibody at 1/200 dilution.| Positive Control | Hela, A549, mouse liver,rat liver, rat spleen tissue lysate, Zebrafish tissue lysates, A431, SK-Br-3, rat skeletal muscle tissue, human fetal skeletal muscle tissue, human pancreas tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
