Application
Fig1: Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.Application
Fig2: ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Aromatase antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Aromatase |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARO antibody ARO1 antibody Aromatase antibody CP19A_HUMAN antibody CPV1 antibody CYAR antibody CYP19 antibody Cyp19a1 antibody CYPXIX antibody Cytochrome P-450AROM antibody Cytochrome P450 19A1 antibody Cytochrome P450, family 19, subfamily A, polypeptide 1 antibody Cytochrome P450, subfamily XIX (aromatization of androgens) antibody Estrogen synthase antibody Estrogen synthetase antibody Flavoprotein linked monooxygenase antibody MGC104309 antibody Microsomal monooxygenase antibody OTTHUMP00000162543 antibody OTTHUMP00000198350 antibody P 450AROM antibody |
| Product description | P450 enzymes constitute a family of monooxygenase enzymes that are involved in the metabolism of a wide array of endogenous and xenobiotic compounds. P450 enzymes can be classified, based on their sequence similarities, into distinct subfamilies, which include CYP1A and CYP2A. Other P450 family members include CYP19, also designated aromatase (P450arom), which catalyzes the conversion of C19 steroids to estrogens in various tissues, including placenta, gonads, adipose tissue, skin and brain. CYP19 expression is controlled by hormonally regulated promoters in different tissues and increased CYP19 activity is associated with familial gynecomastia. Also, a polymorphic allele of CYP19 (repeat (TTTA)12) is present in a majority of breast cancer patients. P450 cholesterol 7α-hydroxylase, CYP7A1, is the rate-limiting enzyme of bile acid synthesis in the liver, and its expression is mediated by the bile acid receptor FXR. CYP27A1 catalyzes vitamin D3 25-hydroxylation and is localized to the mitochondria in kidney and liver. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ARO antibody
ARO1 antibody
Aromatase antibody
CP19A_HUMAN antibody
CPV1 antibody
CYAR antibody
CYP19 antibody
Cyp19a1 antibody
CYPXIX antibody
Cytochrome P-450AROM antibody
Cytochrome P450 19A1 antibody
Cytochrome P450 family 19 subfamily A polypeptide 1 antibody Cytochrome P450 subfamily XIX (aromatization of androgens) antibody Estrogen synthase antibody Estrogen synthetase antibody Flavoprotein linked monooxygenase antibody MGC104309 antibody Microsomal monooxygenase antibody OTTHUMP00000162543 antibody OTTHUMP00000198350 antibody P 450AROM antibody |
|---|---|
| Molecular Weight(MW) | 58 kDa |
| Cellular Localization | Membrane. |
Application
Fig1: Western blot analysis of Aromatase on mouse cerebellum tissue lysate using anti-Aromatase antibody at 1/500 dilution.
Application
Fig2: ICC staining Aromatase in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Aromatase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Aromatase in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Aromatase antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of HepG2 cells with Aromatase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | HepG2, SH-SY-5Y, SK-Br-3, human placenta tissue, mouse cerebellum tissue lysate. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:100 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
