Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | HIF1 alpha |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARNT interacting protein antibody ARNT-interacting protein antibody Basic helix loop helix PAS protein MOP1 antibody Basic-helix-loop-helix-PAS protein MOP1 antibody bHLHe78 antibody Class E basic helix-loop-helix protein 78 antibody HIF 1A antibody HIF 1alpha antibody HIF-1-alpha antibody HIF1 A antibody HIF1 Alpha antibody HIF1 antibody HIF1-alpha antibody HIF1A antibody HIF1A_HUMAN antibody Hypoxia inducible factor 1 alpha antibody Hypoxia inducible factor 1 alpha isoform I.3 antibody Hypoxia inducible factor 1 alpha subunit antibody Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody Hypoxia inducible factor1alpha antibody Hypoxia-inducible factor 1-alpha antibody Member of PAS protein 1 antibody Member of PAS superfamily 1 antibody Member of the PAS Superfamily 1 antibody MOP 1 antibody MOP1 antibody PAS domain-containing protein 8 antibody PASD 8 antibody PASD8 antibody |
| Product description | Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia. |
| Immunogen | Peptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ARNT interacting protein antibody
ARNT-interacting protein antibody
Basic helix loop helix PAS protein MOP1 antibody
Basic-helix-loop-helix-PAS protein MOP1 antibody
bHLHe78 antibody
Class E basic helix-loop-helix protein 78 antibody
HIF 1A antibody
HIF 1alpha antibody
HIF-1-alpha antibody
HIF1 A antibody
HIF1 Alpha antibody
HIF1 antibody
HIF1-alpha antibody
HIF1A antibody
HIF1A_HUMAN antibody
Hypoxia inducible factor 1 alpha antibody
Hypoxia inducible factor 1 alpha isoform I.3 antibody
Hypoxia inducible factor 1 alpha subunit antibody
Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
Hypoxia inducible factor 1 alpha subunit (basic helix loop helix transcription factor) antibody Hypoxia inducible factor1alpha antibody Hypoxia-inducible factor 1-alpha antibody Member of PAS protein 1 antibody Member of PAS superfamily 1 antibody Member of the PAS Superfamily 1 antibody MOP 1 antibody MOP1 antibody PAS domain-containing protein 8 antibody PASD 8 antibody PASD8 antibody |
|---|---|
| Molecular Weight(MW) | 93 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse small intestine tissue lysate, A431, SiHa. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
