Application
Fig1: Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution. Positive control: Lane 1: 293 Lane 2: A431 Lane 3: Jurkat| Product Name | Eg5 [JU83-16] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EG5 antibody HKSP antibody KIF11 antibody KIF11_HUMAN antibody Kinesin family member 11 antibody Kinesin like protein 1 antibody Kinesin-like protein 1 antibody Kinesin-like protein KIF11 antibody Kinesin-like spindle protein HKSP antibody Kinesin-related motor protein Eg5 antibody KNSL1 antibody MCLMR antibody Thyroid receptor-interacting protein 5 antibody TR-interacting protein 5 antibody TRIP-5 antibody TRIP5 antibody |
| Product description | Eukaryotes contain a superfamily of microtubule-based motor proteins comprising kinesin and a number of related proteins that are thought to participate in various forms of intracellular motility, including cell division and organelle transport. KIF11(also known as kinesin family member 11, Eg5 or TRIP5) is a slow, plus-end-directed microtubule-based motor of the BimC kinesin family that is essential for bipolar spindle formation during eukaryotic cell division. When the expression of KIF11 is blocked, centrosome migration halts and cells are arrested in mitosis with monoastral microtubule arrays. KIF11 is phosphorylated on serine during S phase and on both serine and Thr 927 during mitosis, which regulates the association of Eg5 with the spindle apparatus (probably during early prophase). KIF11 is also known to be a member of the thyroid receptor interacting protein (Trip) family, and interacts with the thyroid hormone receptor only in the presence of thyroid hormone. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIP |
| WB:1:500-1:2,000 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | EG5 antibody HKSP antibody KIF11 antibody KIF11_HUMAN antibody Kinesin family member 11 antibody Kinesin like protein 1 antibody Kinesin-like protein 1 antibody Kinesin-like protein KIF11 antibody Kinesin-like spindle protein HKSP antibody Kinesin-related motor protein Eg5 antibody KNSL1 antibody MCLMR antibody Thyroid receptor-interacting protein 5 antibody TR-interacting protein 5 antibody TRIP-5 antibody TRIP5 antibody |
|---|---|
| Molecular Weight(MW) | 119 kDa |
| Cellular Localization | Cytoplasm |
| SwissProt ID | P52732 |
|---|
Application
Fig1: Western blot analysis of Eg5 on different cell lysates using anti-Eg5 antibody at 1/500 dilution. Positive control: Lane 1: 293 Lane 2: A431 Lane 3: Jurkat| Positive Control | 293, A431, Jurkat. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
