Application
Fig1: Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin.Application
Fig5: Flow cytometric analysis of HepG2 cells with GOT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | GOT1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CGI-141 antibody GCT2 antibody Germ cell tumor 2 antibody Golgi transport 1 homolog B antibody Golgi transport 1B antibody GOLT1B antibody GOT1 antibody GOT1B_HUMAN antibody hGOT1a antibody Putative NF-kappa-B-activating protein 470 antibody Vesicle transport protein GOT1B antibody YMR292W antibody |
| Product description | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. |
| Immunogen | Recombinant protein with Human GOT1 aa 1-200. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CGI-141 antibody GCT2 antibody Germ cell tumor 2 antibody Golgi transport 1 homolog B antibody Golgi transport 1B antibody GOLT1B antibody GOT1 antibody GOT1B_HUMAN antibody hGOT1a antibody Putative NF-kappa-B-activating protein 470 antibody Vesicle transport protein GOT1B antibody YMR292W antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P17174 |
|---|
Application
Fig1: Western blot analysis of GOT1 on K562 cell lysate using anti-GOT1 antibody at 1/500 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human heart tissue using anti-GOT1 antibody. Counter stained with hematoxylin.
Application
Fig5: Flow cytometric analysis of HepG2 cells with GOT1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | K562, human liver tissue, human colon tissue, human heart tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
