Application
Fig1: Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.Application
Fig2: ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | DIAPH3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AN antibody AUNA1 antibody Dia2 antibody diap3 antibody DIAP3_HUMAN antibody DIAPH3 antibody Diaphanous homolog 3 (Drosophila) antibody Diaphanous homolog 3 antibody Diaphanous related formin 3 antibody Diaphanous, Drosophila, homolog of, 3 antibody Diaphanous-related formin-3 antibody DKFZp434C0931 antibody DKFZp686A13178 antibody DRF3 antibody FLJ34705 antibody mDia2 antibody NSDAN antibody OTTHUMP00000018480 antibody Protein diaphanous homolog 3 antibody RP11-26P21.1 antibody |
| Product description | DIAPH3 (diaphanous homolog 3), also known as DIAP3, DRF3 or mDia2 of mouse origin, is a 1,193 amino acid member of the formin homology protein family and is required for the correct function of various cellular processes. DIAPH3 binds to both Profilin, a protein involved in cell maintenance, and to the GTP-bound form of Rho (Rho-GTP). Binding to both of these proteins allows DIAPH3 to recruit Profilin to the membrane, in a Rho-dependent manner. At the membrane, DIAPH3 promotes Actin polymerization and is required for stress fiber formation, cytokinesis and transcriptional activation of the serum response factor (SRF). DIAPH3 also regulates Actin dynamics by coupling Src tyrosine kinase (c-Src) and Rho during Actin signaling events. DIAPH3 contains one diaph-anous autoregulatory domain (DAD) and one Rho GTPase-binding domain (GBD). When DAD and GBD are intramolecularly bound, the GBD is occupied and DIAPH3 is inactive. Interruption of the DAD-GBD bond allows the GBD to bind to Rho-GTP, thus activating DIAPH3. Seven isoforms of DIAPH3 exist due to alternative splicing events. |
| Immunogen | Synthetic peptide within human DIAPH3 aa 1-50. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | AN antibody
AUNA1 antibody
Dia2 antibody
diap3 antibody
DIAP3_HUMAN antibody
DIAPH3 antibody
Diaphanous homolog 3 (Drosophila) antibody
Diaphanous homolog 3 antibody
Diaphanous related formin 3 antibody
Diaphanous Drosophila homolog of 3 antibody Diaphanous-related formin-3 antibody DKFZp434C0931 antibody DKFZp686A13178 antibody DRF3 antibody FLJ34705 antibody mDia2 antibody NSDAN antibody OTTHUMP00000018480 antibody Protein diaphanous homolog 3 antibody RP11-26P21.1 antibody |
|---|---|
| Molecular Weight(MW) | 137/80 kDa |
| Cellular Localization | Cytosol. |
| SwissProt ID | Q9NSV4 |
|---|
Application
Fig1: Western blot analysis of DIAPH3 on SiHa cell lysate using anti-DIAPH3 antibody at 1/500 dilution.
Application
Fig2: ICC staining DIAPH3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DIAPH3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining DIAPH3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DIAPH3 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of LOVO cells with DIAPH3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A431, LOVO, SiHa, human kidney tissue, human placenta tissue. |
|---|---|
| Application Notes | ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
