Application
Fig1: Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.Application
Fig2: ICC staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Raptor antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Raptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIAA1303 antibody KOG1 antibody Mip1 antibody P150 target of rapamycin (TOR) scaffold protein antibody p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody P150 target of rapamycin (TOR)-scaffold protein antibody Raptor antibody Regulatory associated protein of mTOR antibody Regulatory associated protein of MTOR complex 1 antibody Regulatory-associated protein of mTOR antibody RPTOR antibody RPTOR_HUMAN antibody |
| Product description | Regulatory associated protein of FRAP, also designated Raptor, is a binding partner for mammalian target of rapamycin kinase (FRAP), and is essential for FRAP signalling in vivo. Raptor binding to FRAP is critical for FRAP-catalysed substrate phosphorylation of 4E-BP1. The raptor-FRAP complex is nutrient-sensitive and is important for a mechanism by which cells coordinate cell growth and size with changing environmental conditions. Raptor serves as a negative regulator of FRAP kinase activity under nutrient-deprived conditions and is an important component in the FRAP pathway. Raptor is highly expressed in skeletal muscle and to a lesser extent in brain, kidney, lung and placenta. |
| Immunogen | Synthetic peptide within human Raptor aa 100-150. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | KIAA1303 antibody KOG1 antibody Mip1 antibody P150 target of rapamycin (TOR) scaffold protein antibody p150 target of rapamycin (TOR) scaffold protein containing WD repeats antibody P150 target of rapamycin (TOR)-scaffold protein antibody Raptor antibody Regulatory associated protein of mTOR antibody Regulatory associated protein of MTOR complex 1 antibody Regulatory-associated protein of mTOR antibody RPTOR antibody RPTOR_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 131 kDa |
| Cellular Localization | Cytoplasm. Lysosome. |
Application
Fig1: Western blot analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.
Application
Fig2: ICC staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Raptor antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, 293T, LOVO, rat brain tissue, human breast tissue, mouse colon tissue, A549. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
