Application
Fig1: Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse placenta tissue Lane 3: MCF-7Application
Fig2: ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.| Product Name | macroH2A.1 [JU22-83] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Core histone macro h2a.1 antibody Core histone macro-H2A.1 antibody H2A histone family member Y antibody H2A.y antibody H2A/y antibody H2AF12M antibody H2AFJ antibody H2afy antibody H2AY_HUMAN antibody Histone H2A.Y antibody Histone macroH2A1 antibody Histone macroH2A1.1 antibody Histone macroH2A1.2 antibody Macroh2a1 antibody MACROH2A1.1 antibody MacroH2A1.2 antibody Medulloblastoma antigen MU MB 50.205 antibody Medulloblastoma antigen MU-MB-50.205 antibody mH2a antibody mH2A1 antibody |
| Product description | Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP-ribose, and may be involved in ADP-ribose-mediated chromatin modulation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Core histone macro h2a.1 antibody Core histone macro-H2A.1 antibody H2A histone family member Y antibody H2A.y antibody H2A/y antibody H2AF12M antibody H2AFJ antibody H2afy antibody H2AY_HUMAN antibody Histone H2A.Y antibody Histone macroH2A1 antibody Histone macroH2A1.1 antibody Histone macroH2A1.2 antibody Macroh2a1 antibody MACROH2A1.1 antibody MacroH2A1.2 antibody Medulloblastoma antigen MU MB 50.205 antibody Medulloblastoma antigen MU-MB-50.205 antibody mH2a antibody mH2A1 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse placenta tissue Lane 3: MCF-7
Application
Fig2: ICC staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.| Positive Control | Hela, mouse placenta tissue lysate, MCF-7, HepG2, PC-3M, human lung cancer tissue, human thyroid grand, human placenta tissue, mouse fallopian tube tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
