Application
Fig1: Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution. Positive control： Lane1: PC-3 Lane2: Mouse testis tissue Lane3: Rat testis tissueApplication
Fig2: ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Rae1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | dJ481F12.3 antibody dJ800J21.1 antibody FLJ30608 antibody Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) antibody Homolog of yeast Rae1 mRNA associated protein of 41 kDa antibody MGC117333 antibody MGC126076 antibody MGC126077 antibody MIG 14 antibody MIG14 antibody Migration inducing gene 14 antibody Mnrp 41 antibody Mnrp41 antibody mRNA associated protein mrnp 41 antibody mRNA binding protein 41 kD antibody mRNA export factor antibody mRNA export protein antibody mRNA-associated protein mrnp 41 antibody MRNP 41 antibody MRNP41 antibody RAE 1 antibody RAE1 (RNA export 1 S.pombe) homolog antibody rae1 antibody RAE1 homolog antibody Rae1 protein homolog antibody RAE1 RNA export 1 homolog (S. pombe) antibody RAE1 RNA export 1 homolog antibody RAE1L_HUMAN antibody RNA export 1 antibody RNA export 1 homolog antibody |
| Product description | Mrnp41 (mRNA-binding protein, 41-KD), also known as Rae1 protein homolog and mRNA export factor, is a 368 amino acid protein that binds mRNA and is involved in nucleocytoplasmic transport. Though characterized in both the nucleus and cytoplasm, mrnp41 is primarily localized to the nuclear pore complex in the nuclear envelope. Mutations in mrnp41 may result in the accumulation of poly(A)-containing mRNA in the nucleus, further supporting the role of mrnp41 as a primary nuclear exporter of mRNA. Along with Nup98, mrnp41 has been shown to regulate E-cadherin, an activating subunit of the anaphase-promoting complex complex, which results in the prevention of securin degradation, therefore suggesting that mrnp41 may play a potential role in maintaining euploidy. Also, during mitosis, both mrnp41 and NuMA have been shown to be required for bipolar spindle formation. |
| Immunogen | Synthetic peptide within human Rae1 aa 330-368. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:500-1,000 ICC:1:50-1:200 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | dJ481F12.3 antibody dJ800J21.1 antibody FLJ30608 antibody Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) antibody Homolog of yeast Rae1 mRNA associated protein of 41 kDa antibody MGC117333 antibody MGC126076 antibody MGC126077 antibody MIG 14 antibody MIG14 antibody Migration inducing gene 14 antibody Mnrp 41 antibody Mnrp41 antibody mRNA associated protein mrnp 41 antibody mRNA binding protein 41 kD antibody mRNA export factor antibody mRNA export protein antibody mRNA-associated protein mrnp 41 antibody MRNP 41 antibody MRNP41 antibody RAE 1 antibody RAE1 (RNA export 1 S.pombe) homolog antibody rae1 antibody RAE1 homolog antibody Rae1 protein homolog antibody RAE1 RNA export 1 homolog (S. pombe) antibody RAE1 RNA export 1 homolog antibody RAE1L_HUMAN antibody RNA export 1 antibody RNA export 1 homolog antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa |
| Cellular Localization | Nucleus. Cytoskeleton.Cytoplasm. |
Application
Fig1: Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution. Positive control： Lane1: PC-3 Lane2: Mouse testis tissue Lane3: Rat testis tissue
Application
Fig2: ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Fig5: Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | PC-3, mouse testis tissue, rat testis tissue, LOVO, MCF-7, PC-3M. |
|---|---|
| Application Notes | WB:1:500-1,000 ICC:1:50-1:200 FC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
