Application
Fig1: Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 Lane 2: NIH-3T3 Lane 3: MCF-7 Lane 4: Rat kidney tissueApplication
Fig2: ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.| Product Name | DDB1 [JU32-35] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCe antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody |
| Product description | Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively. The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light. Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair. XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities. The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFIHCIP |
| WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Damage specific DNA binding protein 1 antibody Damage-specific DNA-binding protein 1 antibody DDB 1 antibody DDB p127 subunit antibody Ddb1 antibody DDB1_HUMAN antibody DDBa antibody DNA damage binding protein 1 antibody DNA damage-binding protein 1 antibody DNA damage-binding protein a antibody HBV X-associated protein 1 antibody UV damaged DNA binding factor antibody UV damaged DNA binding protein 1 antibody UV DDB 1 antibody UV DDB1 antibody UV-damaged DNA-binding factor antibody UV-damaged DNA-binding protein 1 antibody UV-DDB 1 antibody X associated protein 1 antibody XAP 1 antibody XAP-1 antibody XAP1 antibody Xeroderma pigmentosum group E complementing protein antibody Xeroderma pigmentosum group E-complementing protein antibody XPCe antibody XPE antibody XPE BF antibody XPE binding factor antibody XPE-BF antibody XPE-binding factor antibody |
|---|---|
| Molecular Weight(MW) | 127 kDa |
| Cellular Localization | Cytoplasm, Nucleus. |
Application
Fig1: Western blot analysis of DDB1 on different lysates using anti-DDB1 antibody at 1/500 dilution. Positive control: Lane 1: HepG2 Lane 2: NIH-3T3 Lane 3: MCF-7 Lane 4: Rat kidney tissue
Application
Fig2: ICC staining DDB1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining DDB1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin.| Positive Control | HepG2, NIH-3T3, MCF-7, rat kidney tissue lysate, Hela, HUVEC, SH-SY5Y, rat esophagus tissue, human liver cancer tissue, human kidney tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
