Application
Fig1: Western blot analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7Application
Fig2: Western blot analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.Application
Fig3: ICC staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.Application
Fig7: ICC staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.| Product Name | Histone H1.2 [JU43-48] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | H1 histone family member 2 antibody H1.a antibody H12_HUMAN antibody H1F2 antibody H1s-1 antibody HIST1H1C antibody Histone 1 H1c antibody Histone cluster 1 H1c antibody Histone H1.2 antibody Histone H1c antibody Histone H1d antibody Histone H1s-1 antibody MGC3992 antibody |
| Product description | Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCChIP |
| WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | H1 histone family member 2 antibody H1.a antibody H12_HUMAN antibody H1F2 antibody H1s-1 antibody HIST1H1C antibody Histone 1 H1c antibody Histone cluster 1 H1c antibody Histone H1.2 antibody Histone H1c antibody Histone H1d antibody Histone H1s-1 antibody MGC3992 antibody |
|---|---|
| Molecular Weight(MW) | 29 kDa |
| Cellular Localization | Nucleus. Chromosome. |
Application
Fig1: Western blot analysis of Histone H1.2 on different cell lysates using anti-Histone H1.2 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: 293 Lane 3: MCF-7
Application
Fig2: Western blot analysis of Histone H1.2 on Rat liver and Mouse lung tissue lysates using anti-Histone H1.2 antibody at 1/500 dilution.
Application
Fig3: ICC staining Histone H1.2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Histone H1.2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.
Application
Fig7: ICC staining Histone H1.2 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H1.2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins. Counter stained with hematoxylin.| Positive Control | Hela, 293, MCF-7, rat liver tissue lysate, mouse lung tissue lysate, PC-3M, SK-Br-3, human kidney tissue, mouse colon tissue, mouse colon tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
