Application
Fig1: Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.Application
Fig2: ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Heme Oxygenase 1 (HO-1) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.| Product Name | Heme Oxygenase 1 (HO-1) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 32 kD antibody bK286B10 antibody D8Wsu38e antibody heat shock protein 32 kD antibody heat shock protein 32kD antibody Heat shock protein antibody Heme oxygenase (decycling) 1 antibody Heme oxygenase 1 antibody Hemox antibody HMOX 1 antibody Hmox antibody Hmox1 antibody HMOX1_HUMAN antibody HO 1 antibody HO antibody HO-1 antibody HO1 antibody Hsp32 antibody |
| Product description | Heme oxygenases are microsomal enzymes that cleave heme to produce the antioxidant biliverdin, inorganic iron and carbon monoxide (CO). The activity of Heme Oxygenase 1 (HO-1), also designated HSP 32, is highly inducible in response to numerous stimuli, including heme, heavy metals, hormones and oxidative stress. Heme Oxygenase 2, in contrast, appears to be constituitively expressed in mammalian tissues. Heme Oxygenase 2 is involved in the production of carbon monoxide (CO) in brain, where CO is thought to act as a neurotransmitter. The CO signaling system closely parallels the signaling pathway involving nitric oxide, and regulation of the two systems is closely linked. Heme Oxygenase 3 is found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis. A poor heme catalyst, Heme Oxygenase 3 has two heme regulatory motifs that may be involved in heme binding. |
| Immunogen | Synthetic peptide of N-terminal human Heme Oxygenase 1 (HO-1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:500 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | 32 kD antibody bK286B10 antibody D8Wsu38e antibody heat shock protein 32 kD antibody heat shock protein 32kD antibody Heat shock protein antibody Heme oxygenase (decycling) 1 antibody Heme oxygenase 1 antibody Hemox antibody HMOX 1 antibody Hmox antibody Hmox1 antibody HMOX1_HUMAN antibody HO 1 antibody HO antibody HO-1 antibody HO1 antibody Hsp32 antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa |
| Cellular Localization | Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of Heme Oxygenase 1 (HO-1) on mouse spleen tissue lysate using anti-Heme Oxygenase 1 (HO-1) antibody at 1/500 dilution.
Application
Fig2: ICC staining Heme Oxygenase 1 (HO-1) in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Heme Oxygenase 1 (HO-1) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Heme Oxygenase 1 (HO-1) antibody. Counter stained with hematoxylin.| Positive Control | Mouse spleen tissue lysate, 293T, HepG2, human spleen tissue, human placenta tissue, human small intestine tissue, mouse small intestine tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:500 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
