Application
Fig1: Western blot analysis of Transglutaminase 2 on mouse placenta (1) and lung tissue lysates using anti-Transglutaminase 2 antibody at 1/500 dilution.Application
Fig2: Western blot analysis of Transglutaminase 2 on HUVEC cell lysates using anti-Transglutaminase 2 antibody at 1/500 dilution.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Transglutaminase 2 antibody. Counter stained with hematoxylin.| Product Name | Transglutaminase 2 [JU30-02] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ALPHA SUBUNIT antibody C polypeptide antibody EC 2.3.2.13 antibodyepididymis secretory protein Li 45 antibody G alpha h antibody G[a]h antibody Gh CLASS G ALPHA h antibody GNAH antibody GNAH G PROTEIN antibody H POLYPEPTIDE antibody HEL-S-45 antibody Protein glutamine gamma glutamyltransferase 2 antibody Protein-glutamine gamma-glutamyltransferase 2 antibody TG 2 antibody TG(C) antibody TG2 antibody TGase C antibody TGase H antibody TGase-2 antibody TgaseII antibody TGC antibody TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody TGM2 antibody TGM2_HUMAN antibody Tissue transglutaminase antibody Transglutaminase 2 antibody Transglutaminase 2 C polypeptide antibody Transglutaminase C antibody Transglutaminase H antibody Transglutaminase-2 antibody tTG antibody tTGas antibody |
| Product description | Terminally differentiating mammalian epidermal cells acquire an insoluble, 10 to 20 nm thick protein deposit on the intracellular surface of the plasma membrane known as the cross-linked cell envelope (CE). The CE is a component of the epidermis that is generated through formation of disulfide bonds and g-glutamyl-lysine isodipeptide bonds, which are formed by the action of transglutaminases (TGases). TGases are intercellularly localizing, Ca2+-dependent enzymes that catalyze the formation of isopeptide bonds by transferring an amine on to glutaminyl residues, thereby cross-linking glutamine residues and lysine residues in substrate proteins. TGases influence numerous biological processes, including blood coagulation, epidermal differentiation, seminal fluid coagulation, fertilization, cell differentiation and apoptosis. Human keratinocyte transglutaminase (TGase1) is a membrane associated, 817 amino acid protein. Human tissue transglutaminase (TGase2) is an endothelial cell specific, 687 amino acid protein. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIHCIP |
| WB:1:500-1:2,000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | ALPHA SUBUNIT antibody C polypeptide antibody EC 2.3.2.13 antibodyepididymis secretory protein Li 45 antibody G alpha h antibody G[a]h antibody Gh CLASS G ALPHA h antibody GNAH antibody GNAH G PROTEIN antibody H POLYPEPTIDE antibody HEL-S-45 antibody Protein glutamine gamma glutamyltransferase 2 antibody Protein-glutamine gamma-glutamyltransferase 2 antibody TG 2 antibody TG(C) antibody TG2 antibody TGase C antibody TGase H antibody TGase-2 antibody TgaseII antibody TGC antibody TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody TGM2 antibody TGM2_HUMAN antibody Tissue transglutaminase antibody Transglutaminase 2 antibody Transglutaminase 2 C polypeptide antibody Transglutaminase C antibody Transglutaminase H antibody Transglutaminase-2 antibody tTG antibody tTGas antibody |
|---|---|
| Molecular Weight(MW) | 77 kDa |
| Cellular Localization | Cytosol. Plasma Membrane. |
Application
Fig1: Western blot analysis of Transglutaminase 2 on mouse placenta (1) and lung tissue lysates using anti-Transglutaminase 2 antibody at 1/500 dilution.
Application
Fig2: Western blot analysis of Transglutaminase 2 on HUVEC cell lysates using anti-Transglutaminase 2 antibody at 1/500 dilution.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Transglutaminase 2 antibody. Counter stained with hematoxylin.| Positive Control | HUVEC, mouse placenta tissue lysate, mouse lung tissue lysate, human placenta tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
