Application
Fig1: Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.Application
Fig2: ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.| Product Name | ASK1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis signal regulating kinase 1 antibody Apoptosis signal-regulating kinase 1 antibody ASK 1 antibody ASK-1 antibody ASK1 antibody M3K5 antibody M3K5_HUMAN antibody MAP/ERK kinase kinase 5 antibody MAP3K5 antibody MAPK/ERK kinase kinase 5 antibody MAPKKK5 antibody MEK kinase 5 antibody MEKK 5 antibody MEKK5 antibody Mitogen activated protein kinase kinase kinase 5 antibody Mitogen-activated protein kinase kinase kinase 5 antibody |
| Product description | Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways. |
| Immunogen | Synthetic peptide of C-terminal human ASK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Alternative Names | Apoptosis signal regulating kinase 1 antibody Apoptosis signal-regulating kinase 1 antibody ASK 1 antibody ASK-1 antibody ASK1 antibody M3K5 antibody M3K5_HUMAN antibody MAP/ERK kinase kinase 5 antibody MAP3K5 antibody MAPK/ERK kinase kinase 5 antibody MAPKKK5 antibody MEK kinase 5 antibody MEKK 5 antibody MEKK5 antibody Mitogen activated protein kinase kinase kinase 5 antibody Mitogen-activated protein kinase kinase kinase 5 antibody |
|---|---|
| Molecular Weight(MW) | 155 kDa |
| Cellular Localization | Cytoplasm. Endoplasmic reticulum. |
Application
Fig1: Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.
Application
Fig2: ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.| Positive Control | Human skeletal muscle tissue lysate, human colon cancer tissue, mouse testis tissue, PANC-1. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
