Application
Fig1: Western blot analysis of Staufen on K562 cell using anti-Staufen antibody at 1/1,000 dilution.Application
Fig2: ICC staining Staufen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Staufen in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Staufen in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig 5: Flow cytometric analysis of SH-SY5Y cells with Staufen antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | Staufen [JU30-85] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Double stranded RNA binding protein Staufen homolog 1 antibody Double stranded RNA binding protein Staufen homolog antibody Double-stranded RNA-binding protein Staufen homolog 1 antibody FLJ25010 antibody MGC124588 antibody PPP1R150 antibody STAU antibody STAU1 antibody STAU1_HUMAN antibody staufen antibody Staufen RNA binding protein (Drosophila) antibody Staufen RNA binding protein homolog 1 antibody Staufen, Drosophila, homolog of, 1 antibody Staufen, RNA binding protein, homolog 1 (Drosophila) antibody staufen-like antibody |
| Product description | STAU1 (staufen, RNA binding protein, homolog 1) is a 577 amino acid protein that contains three double-stranded RNA-binding domains and is a mammalian homolog of Staufen, a Drosophila protein that is involved in mRNA transport during oogenesis and zygotic development. Localized to the rough endoplasmic reticulum (RER) and expressed in a variety of tissues, including heart, brain, liver, lung, pancreas, kidney and placenta, STAU1 binds to both Tubulin and double-stranded RNA and is thought to play an important role in mRNA transport from the microtubule network to the RER. Additionally, STAU1 may be involved in cross-linking cytoskeletal components with RNA, an event that is important for proper mRNA positioning during translation. Alternative splicing of the STAU1 gene yields two STAU1 isoforms, designated short and long. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICC/IFFC |
| WB:1:500-1:2,000 ICC/IF:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Double stranded RNA binding protein Staufen homolog 1 antibody
Double stranded RNA binding protein Staufen homolog antibody
Double-stranded RNA-binding protein Staufen homolog 1 antibody
FLJ25010 antibody
MGC124588 antibody
PPP1R150 antibody
STAU antibody
STAU1 antibody
STAU1_HUMAN antibody
staufen antibody
Staufen RNA binding protein (Drosophila) antibody
Staufen RNA binding protein homolog 1 antibody
Staufen Drosophila homolog of 1 antibody Staufen RNA binding protein homolog 1 (Drosophila) antibody staufen-like antibody |
|---|---|
| Molecular Weight(MW) | 63 kDa |
| Cellular Localization | Cytoplasm. Rough endoplasmic reticulum. |
Application
Fig1: Western blot analysis of Staufen on K562 cell using anti-Staufen antibody at 1/1,000 dilution.
Application
Fig2: ICC staining Staufen in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Staufen in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Staufen in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig 5: Flow cytometric analysis of SH-SY5Y cells with Staufen antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | K562, HepG2, LOVO, SH-SY5Y. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC/IF:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
