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Fig1: Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution.
Positive control:
Lane1: MCF-7
Lane2: Mouse marrow
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Fig2: ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig3: ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig4: ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
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Fig6: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
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Fig8: Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.