Application
Fig1: Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.| Product Name | Anti-sfGFP antibdody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning. |
| Immunogen | Synthetic peptide RDHMVLHEYVNAAGITC linked to KLH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1,000 |
|
| Concentration | 1mg/ml |
Application
Fig1: Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.| Positive Control | GFP11-tagged fusion protein. |
|---|---|
| Application Notes | WB:1:1,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
