Application
Fig1: Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.| Product Name | mAID-tag |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag. |
| Immunogen | Synthetic peptide of Auxin Inducible Degron Internal region (mini-AID-tag). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1,000-1:5,000 |
|
| Concentration | 1mg/ml |
Application
Fig1: Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.| Positive Control | Mini-AID-tagged fusion protein. |
|---|---|
| Application Notes | WB:1:1,000-1:5,000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
