Application
Fig1: Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.Application
Fig2: ICC staining TYK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TYK2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- TYK2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- TYK2 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of MCF-7 cells with TYK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Tyk2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | JTK 1 antibody JTK1 antibody Non receptor tyrosine protein kinase 2 antibody Non receptor tyrosine protein kinase TYK2 antibody Non-receptor tyrosine-protein kinase TYK2 antibody OTTHUMP00000232745 antibody OTTHUMP00000232746 antibody OTTHUMP00000232748 antibody Protein Tyrosine Kinase 2 antibody TYK 2 antibody Tyk2 antibody TYK2_HUMAN antibody Tyrosine kinase 2 antibody |
| Product description | TYK2 belongs to the family of non-receptor janus tyrosine kinases, which regulate a spectrum of cellular functions occurring downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, recruit STAT transcription factors to the cytokine receptor where TYK2 is associated. TYK2 then phosphorylates proximal STAT factors, which subsequently dimerize, translocate to the nucleus, and bind to cis elements upstream of target gene promoters to regulating transcription. The canonical JAK-STAT pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival, and host resistance to pathogens. Cytokine induced pro-inflammatory responses are attractive targets for anti-inflammatory therapies, specifically at the level of Jak-STAT signaling. |
| Immunogen | Recombinant protein within human TYK2 aa 400-600. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | JTK 1 antibody JTK1 antibody Non receptor tyrosine protein kinase 2 antibody Non receptor tyrosine protein kinase TYK2 antibody Non-receptor tyrosine-protein kinase TYK2 antibody OTTHUMP00000232745 antibody OTTHUMP00000232746 antibody OTTHUMP00000232748 antibody Protein Tyrosine Kinase 2 antibody TYK 2 antibody Tyk2 antibody TYK2_HUMAN antibody Tyrosine kinase 2 antibody |
|---|---|
| Molecular Weight(MW) | 134 kDa |
| Cellular Localization | Cytoskeleton. Nucleus. Cytosol. Plasma membrane. |
Application
Fig1: Western blot analysis of TYK2 on MCF-7 cell lysate using anti-TYK2 antibody at 1/500 dilution.
Application
Fig2: ICC staining TYK2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TYK2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- TYK2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti- TYK2 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of MCF-7 cells with TYK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; green). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, rat kidney tissue, human liver cancer tissue, human colon tissue. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
