Application
Fig1: Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.Application
Fig2: ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).Application
Fig3: ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).Application
Fig4: ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of HepG2 cells with UQCRC2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | UQCRC2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Complex III subunit 2 antibody Core protein II antibody Cytochrome b c1 complex subunit 2 mitochondrial antibody Cytochrome b-c1 complex subunit 2 antibody EC 1.10.2.2 antibody MC3DN5 antibody mitochondrial antibody QCR2 antibody QCR2_HUMAN antibody Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody Ubiquinol cytochrome c reductase core protein II antibody Ubiquinol-cytochrome-c reductase complex core protein 2 antibody UQCR2 antibody Uqcrc2 antibody |
| Product description | Cytochrome c is a well characterized, mobile electron transport protein that is essential to energy conversion in all aerobic organisms. Cytochrome b associates with cytochrome c subunit 1 and the Rieske protein to form complex III (also designated cytochrome bc1 complex), which is involved in cellular respiration. Ubiquinol cytochrome c reductase (UQCRFS1), also referred to as Rieske iron-sulfur protein, represents an important subunit of complex III of the mitochondrial respiratory chain that transfers electrons from ubiquinol to cytochrome c. The UQCRFS1 complex is made up of 3 respiratory subunits (cytochrome b, cytochrome c1, Rieske protein), 2 core proteins, and 6 low-molecular weight proteins. Ubiquinol cytochrome-c reductase complex core protein 2 (UQCRC2) represents one of the core proteins of UQCRFS1, and it is required for the assembly of the complex. |
| Immunogen | Recombinant protein within human UQCRC2 aa 100-350. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:1,000-2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Complex III subunit 2 antibody Core protein II antibody Cytochrome b c1 complex subunit 2 mitochondrial antibody Cytochrome b-c1 complex subunit 2 antibody EC 1.10.2.2 antibody MC3DN5 antibody mitochondrial antibody QCR2 antibody QCR2_HUMAN antibody Ubiquinol cytochrome c reductase complex core protein 2 mitochondrial antibody Ubiquinol cytochrome c reductase core protein II antibody Ubiquinol-cytochrome-c reductase complex core protein 2 antibody UQCR2 antibody Uqcrc2 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1: Western blot analysis of UQCRC2 on rat heart tissue lysate using anti-UQCRC2 antibody at 1/5,000 dilution.
Application
Fig2: ICC staining UQCRC2 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
Application
Fig3: ICC staining UQCRC2 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
Application
Fig4: ICC staining UQCRC2 in SiHa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. DAPI was used to stain the cell nuclei (blue).
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-UQCRC2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of HepG2 cells with UQCRC2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Rat heart tissue, human liver tissue, mouse brain tissue, HepG2. |
|---|---|
| Application Notes | WB:1:1,000-2,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
