Application
Fig1: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution. Positive control： Lane1: 293T Lane2: Daudi Lane3: Mouse testis Lane4: Rat brainApplication
Fig2: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | USP13 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Deubiquitinating enzyme 13 antibody Isopeptidase T 3 antibody Isopeptidase T-3 antibody Isopeptidase T3 antibody ISOT 3 antibody ISOT-3 antibody ISOT3 antibody Ubiquitin carboxyl terminal hydrolase 13 antibody Ubiquitin carboxyl-terminal hydrolase 13 antibody Ubiquitin specific peptidase 13 (isopeptidase T3) antibody Ubiquitin specific peptidase 13 antibody Ubiquitin specific processing protease 13 antibody Ubiquitin specific protease 13 antibody Ubiquitin thioesterase 13 antibody Ubiquitin thiolesterase 13 antibody Ubiquitin-specific-processing protease 13 antibody UBP13 antibody UBP13_HUMAN antibody USP 13 antibody USP13 antibody |
| Product description | The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub and Ub-like molecules to specific protein substrates. Through the use of a wide range of enzymes that can add or remove ubiquitin, the Ub pathway controls many intracellular processes such as signal transduction, transcriptional activation and cell cycle progression. USP13 (ubiquitin specific peptidase 13), also known as ISOT3 (Isopeptidase T-3), is an 863 amino acid protein that belongs to the peptidase C19 family and contains one UBP-type zinc finger and two UBA domains. Highly expressed in testicular and ovarian tissue, USP13 functions to catalyze the water-dependent conversion of a ubiquitin C-terminal thioester to a thiol and a free ubiquitin. |
| Immunogen | Recombinant protein within human USP13 aa 550-800. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFCIHC |
| WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:100-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | Deubiquitinating enzyme 13 antibody Isopeptidase T 3 antibody Isopeptidase T-3 antibody Isopeptidase T3 antibody ISOT 3 antibody ISOT-3 antibody ISOT3 antibody Ubiquitin carboxyl terminal hydrolase 13 antibody Ubiquitin carboxyl-terminal hydrolase 13 antibody Ubiquitin specific peptidase 13 (isopeptidase T3) antibody Ubiquitin specific peptidase 13 antibody Ubiquitin specific processing protease 13 antibody Ubiquitin specific protease 13 antibody Ubiquitin thioesterase 13 antibody Ubiquitin thiolesterase 13 antibody Ubiquitin-specific-processing protease 13 antibody UBP13 antibody UBP13_HUMAN antibody USP 13 antibody USP13 antibody |
|---|---|
| Molecular Weight(MW) | 97 kDa |
| Cellular Localization | Nucleus. Cytosol. |
Application
Fig1: Western blot analysis of USP13 on different lysates using anti-USP13 antibody at 1/1,000 dilution. Positive control： Lane1: 293T Lane2: Daudi Lane3: Mouse testis Lane4: Rat brain
Application
Fig2: ICC staining USP13 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining USP13 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Flow cytometric analysis of A431 cells with USP13 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | 293T, Daudi, mouse testis tissue, rat brain tissue, A431, MG-63. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 IHC:1:100-1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
