Application
Fig1: Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution. Lane 1 : Raji cell lysates Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)Application
Fig2: ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Orai3 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MGC13024 antibody Orai 3 antibody ORAI calcium release activated calcium modulator 3 antibody Orai3 antibody ORAI3_HUMAN antibody Protein orai 3 antibody Protein orai-3 antibody TMEM 142C antibody TMEM142C antibody Transmembrane protein 142C antibody |
| Product description | ORAI3 (ORAI Calcium Release-Activated Calcium Modulator 3) is a Protein Coding gene. Among its related pathways are Calcium signaling pathway. Gene Ontology (GO) annotations related to this gene include store-operated calcium channel activity. An important paralog of this gene is ORAI2. |
| Immunogen | Synthetic peptide within human Orai-3 aa 10-50. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | MGC13024 antibody Orai 3 antibody ORAI calcium release activated calcium modulator 3 antibody Orai3 antibody ORAI3_HUMAN antibody Protein orai 3 antibody Protein orai-3 antibody TMEM 142C antibody TMEM142C antibody Transmembrane protein 142C antibody |
|---|---|
| Molecular Weight(MW) | 31 kDa |
| Cellular Localization | Membrane. Multi-pass membrane protein. |
Application
Fig1: Western blot analysis of Orai3 using anti-Orai3 antibody at 1/500 dilution. Lane 1 : Raji cell lysates Lane 2 : Raji cell lysates(anti-Orai3 antibody+ immunizing peptide)
Application
Fig2: ICC staining Orai3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Orai3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Orai3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Orai3 antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of A549 cells with Orai3 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Raji, A549, MG-63, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human kidney tissue, human placenta tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
