Application
Fig1: Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.Application
Fig2: ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.Application
Fig6: Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Cacng4 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CACNG4 antibody Calcium channel voltage dependent gamma subunit 4 antibody CCG4_HUMAN antibody Neuronal voltage gated calcium channel gamma 4 subunit antibody Neuronal voltage-gated calcium channel gamma-4 subunit antibody TARP gamma-4 antibody Transmembrane AMPAR regulatory protein gamma-4 antibody Voltage dependent calcium channel gamma 4 subunit antibody Voltage-dependent calcium channel gamma-4 subunit antibody |
| Product description | Regulates the activity of L-type calcium channels that contain CACNA1C as pore-forming subunit. Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs), including GRIA1 and GRIA4. Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization and by mediating their resensitization. |
| Immunogen | Synthetic peptide within rat Cacng4 aa 150-250. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | CACNG4 antibody Calcium channel voltage dependent gamma subunit 4 antibody CCG4_HUMAN antibody Neuronal voltage gated calcium channel gamma 4 subunit antibody Neuronal voltage-gated calcium channel gamma-4 subunit antibody TARP gamma-4 antibody Transmembrane AMPAR regulatory protein gamma-4 antibody Voltage dependent calcium channel gamma 4 subunit antibody Voltage-dependent calcium channel gamma-4 subunit antibody |
|---|---|
| Molecular Weight(MW) | 37 kDa |
| Cellular Localization | Cell membrane. |
Application
Fig1: Western blot analysis of Cacng4 on MCF-7 cell lysate using anti-Cacng4 antibody at 1/500 dilution.
Application
Fig2: ICC staining Cacng4 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cacng4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti- Cacng4 antibody. Counter stained with hematoxylin.
Application
Fig6: Flow cytometric analysis of SH-SY5Y cells with Cacng4 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | MCF-7, SH-SY-5Y, rat testis tissue, rat brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:100 IHC:1:50 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
