Application
Fig1: Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.Application
Fig2: ICC staining TRP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining TRP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TRP1 antibody. Counter stained with hematoxylin.| Product Name | TRP1 [JU36-48] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5 antibody 5,6 dihydroxyindole 2 carboxylic acid oxidase antibody 6-dihydroxyindole-2-carboxylic acid oxidase antibody b-PROTEIN antibody CAS2 antibody Catalase B antibody CATB antibody DHICA oxidase antibody Glycoprotein 75 antibody GP75 antibody Melanoma antigen gp75 antibody OCA3 antibody TRP antibody TRP-1 antibody TRP1 antibody Tyrosinase related protein 1 antibody Tyrosinase-related protein 1 antibody TYRP antibody TYRP1 antibody TYRP1_HUMAN antibody TYRRP antibody |
| Product description | Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIHCIP |
| WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 IP:1:10-1:50 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 5 antibody
5 6 dihydroxyindole 2 carboxylic acid oxidase antibody 6-dihydroxyindole-2-carboxylic acid oxidase antibody b-PROTEIN antibody CAS2 antibody Catalase B antibody CATB antibody DHICA oxidase antibody Glycoprotein 75 antibody GP75 antibody Melanoma antigen gp75 antibody OCA3 antibody TRP antibody TRP-1 antibody TRP1 antibody Tyrosinase related protein 1 antibody Tyrosinase-related protein 1 antibody TYRP antibody TYRP1 antibody TYRP1_HUMAN antibody TYRRP antibody |
|---|---|
| Molecular Weight(MW) | 72 kDa |
| Cellular Localization | Membrane. |
| SwissProt ID | P17643 |
|---|
Application
Fig1: Western blot analysis of TRP1 on melanoma tissue lysate using anti-TRP1 antibody at 1/500 dilution.
Application
Fig2: ICC staining TRP1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining TRP1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-TRP1 antibody. Counter stained with hematoxylin.| Positive Control | A431, HUVEC, human skin tissue, Melanoma tissue lysate. |
|---|---|
| Application Notes | WB:1:500-1:2,000 ICC:1:50-1:100 IHC:1:50-1:200 IP:1:10-1:50 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
