Application
Fig1: Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution. Positive control： Lane1: U937 Lane2: SK-Br-3Application
Fig2: ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Vitamin D3 Receptor |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1 25 dihydroxyvitamin D3 receptor antibody 1 antibody 1,25 dihydroxyvitamin D3 receptor antibody 1,25-@dihydroxyvitamin D3 receptor antibody 25-dihydroxyvitamin D3 receptor antibody Member 1 antibody NR1I1 antibody Nuclear receptor subfamily 1 group I member 1 antibody PPP1R163 antibody Protein phosphatase 1, regulatory subunit 163 antibody VDR antibody VDR_HUMAN antibody Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody Vitamin D hormone receptor antibody Vitamin D nuclear receptor variant 1 antibody Vitamin D receptor antibody Vitamin D3 receptor antibody |
| Product description | The active metabolite of vitamin D modulates the expression of a wide variety of genes in a developmentally specific manner. This secosteroid hormone can up- or downregulate the expression of genes involved in a diverse array of responses such as proliferation, differentiation and calcium homeostasis. 1,25-(OH)2-vitamin D3 exerts its effects through interaction with the vitamin D receptor (VDR), a member of the superfamily of hormone-activated nuclear receptors. In its ligand-bound state, the VDR forms heterodimers with the 9-cis retinoic acid receptor, RXR, and affects gene expression by binding specific DNA sequences known as hormone response elements, or HREs. In addition to regulating the above-mentioned cellular responses, 1,25-(OH)2-vitamin D3 exhibits antiproliferative properties in osteosarcoma, melanoma, colon carcinoma and breast carcinoma cells. |
| Immunogen | Recombinant protein with human Vitamin D Receptor aa 100-300. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCFC |
| WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Alternative Names | 1 25 dihydroxyvitamin D3 receptor antibody
1 antibody
1 25 dihydroxyvitamin D3 receptor antibody 1 25-@dihydroxyvitamin D3 receptor antibody 25-dihydroxyvitamin D3 receptor antibody Member 1 antibody NR1I1 antibody Nuclear receptor subfamily 1 group I member 1 antibody PPP1R163 antibody Protein phosphatase 1 regulatory subunit 163 antibody VDR antibody VDR_HUMAN antibody Vitamin D (1 25- dihydroxyvitamin D3) receptor antibody Vitamin D hormone receptor antibody Vitamin D nuclear receptor variant 1 antibody Vitamin D receptor antibody Vitamin D3 receptor antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Nucleus. |
Application
Fig1: Western blot analysis of Vitamin D Receptor on different cell lysate using anti-Vitamin D Receptor antibody at 1/2,000 dilution. Positive control： Lane1: U937 Lane2: SK-Br-3
Application
Fig2: ICC staining Vitamin D Receptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Vitamin D Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Vitamin D Receptor in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of LOVO cells with Vitamin D Receptor antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | U937, SK-Br-3, LOVO, MCF-7, PC-3M, rat skin tissue. |
|---|---|
| Application Notes | WB:1:1,000-1:2,000 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
