Application
Fig1: ICC staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.| Product Name | PCK1 [JU84-39] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | cytosolic [GTP] antibody GTP antibody PCK1 antibody PCKGC_HUMAN antibody PEP carboxykinase antibody PEPCK-C antibody PEPCK1 antibody PEPCKC antibody Phosphoenolpyruvate carboxykinase 1 (soluble) antibody Phosphoenolpyruvate carboxykinase 1 antibody Phosphoenolpyruvate carboxykinase antibody Phosphoenolpyruvate carboxykinase, cytosolic [GTP] antibody Phosphoenolpyruvate carboxykinase, cytosolic antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody |
| Product description | Normal adjustment to changes in blood glucose levels depends on insulin signaling as well as enzymes involved in the regulation of gluconeogenesis. Pathological changes to this process are central to the type 2 diabetes phenotype. Phosphoenolpyruvate carboxykinase (PEPCK) plays an important role in this process by stimulating hepatic glucose production. PEPCK expression increases in response to glucagon and glucocorticoids, while insulin suppresses expression. Modulation of the signals governing PEPCK levels present a potential therapeutic approach to the treatment of insulin resistance and consequently obesity. The cytosolic form of PEPCK, known as PEPCK-C, and the mitochondrial form, known as PEPCK-M, are encoded by two different nuclear genes in mouse, human and chicken. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | ICCIHCWB |
| ICC:1:50-1:200 IHC:1:50-1:200 WB:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | cytosolic [GTP] antibody
GTP antibody
PCK1 antibody
PCKGC_HUMAN antibody
PEP carboxykinase antibody
PEPCK-C antibody
PEPCK1 antibody
PEPCKC antibody
Phosphoenolpyruvate carboxykinase 1 (soluble) antibody
Phosphoenolpyruvate carboxykinase 1 antibody
Phosphoenolpyruvate carboxykinase antibody
Phosphoenolpyruvate carboxykinase cytosolic [GTP] antibody Phosphoenolpyruvate carboxykinase cytosolic antibody Phosphoenolpyruvate carboxylase antibody Phosphopyruvate carboxylase antibody |
|---|---|
| Molecular Weight(MW) | 69 kDa |
| Cellular Localization | Cytoplasm. |
| SwissProt ID | P35558 |
|---|
Application
Fig1: ICC staining PCK1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining PCK1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining PCK1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PCK1 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PCK1 antibody. Counter stained with hematoxylin.| Positive Control | 293T, HepG2, SH-SY5Y, human liver tissue, human kidney tissue. |
|---|---|
| Application Notes | ICC:1:50-1:200 IHC:1:50-1:200 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
