Application
Fig1: Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.Application
Fig2: ICC staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Flow cytometric analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | NR0B1 [JU33-05] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AHC antibody AHCH antibody AHX antibody DAX 1 antibody DAX1 antibody Dosage sensitive sex reversal antibody DSS antibody DSS AHC critical region on the X chromosome protein 1 antibody DSS-AHC critical region on the X chromosome protein 1 antibody GTD antibody HHG antibody Nr0b1 antibody NR0B1_HUMAN antibody NROB1 antibody Nuclear hormone receptor antibody Nuclear receptor 0B1 antibody Nuclear receptor DAX 1 antibody Nuclear receptor DAX-1 antibody Nuclear receptor DAX1 antibody Nuclear receptor subfamily 0 group B member 1 antibody SRXY2 antibody |
| Product description | Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCFC |
| WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | AHC antibody AHCH antibody AHX antibody DAX 1 antibody DAX1 antibody Dosage sensitive sex reversal antibody DSS antibody DSS AHC critical region on the X chromosome protein 1 antibody DSS-AHC critical region on the X chromosome protein 1 antibody GTD antibody HHG antibody Nr0b1 antibody NR0B1_HUMAN antibody NROB1 antibody Nuclear hormone receptor antibody Nuclear receptor 0B1 antibody Nuclear receptor DAX 1 antibody Nuclear receptor DAX-1 antibody Nuclear receptor DAX1 antibody Nuclear receptor subfamily 0 group B member 1 antibody SRXY2 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
| SwissProt ID | P51843 |
|---|
Application
Fig1: Western blot analysis of NR0B1 on A549 cell lysate using anti-NR0B1 antibody at 1/500 dilution.
Application
Fig2: ICC staining NR0B1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NR0B1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NR0B1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Flow cytometric analysis of A549 cells with NR0B1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, Hela, SH-SY-5Y. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
