Application
Fig1: Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis Lane 2: SiHa Lane 3: SK-BR-3 Lane 4: PC-3MApplication
Fig2: ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Cellular Apoptosis Susceptibility [JU34-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CAS antibody Cellular apoptosis susceptibility protein antibody Chromosome segregation 1 (yeast homolog) like antibody Chromosome segregation 1 Like antibody Chromosome segregation 1 like protein antibody Chromosome segregation 1-like protein antibody Chromosome segregation gene CSE1 antibody CSE 1 antibody CSE 1 chromosome segregation 1 like antibody CSE 1 chromosome segregation 1 like protein antibody CSE 1L antibody CSE1 antibody CSE1 chromosome segregation 1 like (yeast) antibody CSE1 chromosome segregation 1 like antibody CSE1 chromosome segregation 1 like protein antibody CSE1L antibody Exp 2 antibody Exp2 antibody Exportin 2 antibody Exportin-2 antibody Exportin2 antibody Importin alpha re exporter antibody Importin-alpha re-exporter antibody MGC117283 antibody MGC130036 antibody MGC130037 antibody XPO 2 antibody XPO2 antibody XPO2_HUMAN antibody |
| Product description | Cellular apoptosis susceptibility protein (CAS), also called Exportin 2, is a 971 amino acid member of the CSE1 family. CAS mediates Importin α re-export from the nucleus to the cytoplasm after import substrates have been released into the nucleoplasm. In the nucleus, CAS binds cooperatively to Importin α and to the GTPase Ran in its GTP-bound (active) form. This complex binds to nucleoporins as it docks to the nuclear pore complex. Once in the cytoplasm, the complex dissociates and Importin α is released and CAS returns to the nuclear compartment and the process begins anew. CAS can be detected highly in proliferating cells. Three isoforms of CAS have been named due to alternative splicing. Isoform 1 is the full length, 971 amino acid protein. Isoform 2 contains an alternative sequence for amino acids 190-195 and is missing amino acids 196-971. Isoform 3 contains an alternative sequence for amino acids 943-945 and is missing amino acids 946-971. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | CAS antibody Cellular apoptosis susceptibility protein antibody Chromosome segregation 1 (yeast homolog) like antibody Chromosome segregation 1 Like antibody Chromosome segregation 1 like protein antibody Chromosome segregation 1-like protein antibody Chromosome segregation gene CSE1 antibody CSE 1 antibody CSE 1 chromosome segregation 1 like antibody CSE 1 chromosome segregation 1 like protein antibody CSE 1L antibody CSE1 antibody CSE1 chromosome segregation 1 like (yeast) antibody CSE1 chromosome segregation 1 like antibody CSE1 chromosome segregation 1 like protein antibody CSE1L antibody Exp 2 antibody Exp2 antibody Exportin 2 antibody Exportin-2 antibody Exportin2 antibody Importin alpha re exporter antibody Importin-alpha re-exporter antibody MGC117283 antibody MGC130036 antibody MGC130037 antibody XPO 2 antibody XPO2 antibody XPO2_HUMAN antibody |
|---|---|
| Molecular Weight(MW) | 110 kDa |
| Cellular Localization | Cytoplasm. Nucleus. |
Application
Fig1: Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis Lane 2: SiHa Lane 3: SK-BR-3 Lane 4: PC-3M
Application
Fig2: ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Mouse testis tissue, SiHa, SK-BR-3, PC-3M, LOVO, SH-SY5Y, human colon cancer tissue, LOVO. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
