Application
Fig1: Western blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with ACCN2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | ACCN2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACCN2 antibody ACCN2_HUMAN antibody Acid sensing ion channel 1 antibody Acid sensing ion channel 1a protein antibody Acid sensing ion channel antibody Acid-sensing ion channel 1 antibody AI843610 antibody Amiloride sensitive cation channel 2 neuronal antibody Amiloride-sensitive cation channel 2 antibody ASIC antibody ASIC1 antibody ASIC1A antibody B530003N02Rik antibody BNaC2 antibody Brain sodium channel 2 antibody Cation channel amiloride sensitive neuronal 2 antibody hBNaC2 antibody neuronal antibody |
| Product description | Isoform 2 and isoform 3 function as proton-gated sodium channels; they are activated by a drop of the extracellular pH and then become rapidly desensitized. The channel generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Isoform 2 can also transport potassium, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 can also transport calcium ions. Mediates glutamate-independent Ca2+ entry into neurons upon acidosis. This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.Isoform 1 does not display proton-gated cation channel activity. |
| Immunogen | Synthetic peptide within human ACCN2 aa 460-500. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | ACCN2 antibody ACCN2_HUMAN antibody Acid sensing ion channel 1 antibody Acid sensing ion channel 1a protein antibody Acid sensing ion channel antibody Acid-sensing ion channel 1 antibody AI843610 antibody Amiloride sensitive cation channel 2 neuronal antibody Amiloride-sensitive cation channel 2 antibody ASIC antibody ASIC1 antibody ASIC1A antibody B530003N02Rik antibody BNaC2 antibody Brain sodium channel 2 antibody Cation channel amiloride sensitive neuronal 2 antibody hBNaC2 antibody neuronal antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa |
| Cellular Localization | Plasma membrane. |
Application
Fig1: Western blot analysis of ACCN2 on SH-SY-5Y cell lysate using anti-ACCN2 antibody at 1/2,000 dilution.
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACCN2 antibody. Counter stained with hematoxylin.
Application
Fig4: Flow cytometric analysis of SH-SY-5Y cells with ACCN2 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SH-SY-5Y, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | WB:1:500-1:2,000 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
