Application
Fig1: Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: HL-60 Lane 3: SH-SY-5YApplication
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin.Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Product Name | ILF2 [JU50-33] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | HGNC:6037 antibody ILF 2 antibody ilf2 antibody ILF2_HUMAN antibody Interleukin enhancer binding factor 2 antibody Interleukin enhancer binding factor 2, 45kDa antibody Interleukin enhancer-binding factor 2 antibody MGC8391 antibody NF 45 antibody NF45 antibody Nuclear factor of activated T cells 45 kDa antibody Nuclear factor of activated T-cells 45 kDa antibody PRO3063 antibody |
| Product description | NF45 (ILF2, nuclear factor of activated T cells 45 kDa) is a transcription factor that interacts with NF90 (ILF3, DRBP76) to regulate gene expression. NF45 and NF90 are proteins that belong to the double-stranded RNA-binding protein family and both are substrates for the dsRNA-activated protein kinase, PKR. The NF45/NF90 heterodimer is mainly involved in regulating IL-2 expression by binding to the antigen receptor response element (ARRE) target sequence of the IL-2 enhancer. In neuronal cells, the NF45/NF90 heterodimer can repress human rhinovirus type 2 replication by binding to a 5' untranslated region of the viral RNA that encodes the internal ribosome entry site (IRES). |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBIPIHCFC |
| WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:20 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | HGNC:6037 antibody
ILF 2 antibody
ilf2 antibody
ILF2_HUMAN antibody
Interleukin enhancer binding factor 2 antibody
Interleukin enhancer binding factor 2 45kDa antibody Interleukin enhancer-binding factor 2 antibody MGC8391 antibody NF 45 antibody NF45 antibody Nuclear factor of activated T cells 45 kDa antibody Nuclear factor of activated T-cells 45 kDa antibody PRO3063 antibody |
|---|---|
| Molecular Weight(MW) | 43 kDa |
| Cellular Localization | Nucleus. Cytoplasm. |
Application
Fig1: Western blot analysis of ILF2 on different cell lysates using anti-ILF2 antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis tissue Lane 2: HL-60 Lane 3: SH-SY-5Y
Application
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-ILF2 antibody. Counter stained with hematoxylin.
Application
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ILF2 antibody. Counter stained with hematoxylin.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ILF2 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ILF2 antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ILF2 antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of SH-SY-5Y cells with ILF2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.| Positive Control | HL-60, SH-SY-5Y, rat brain tissue, human tonsil tissue, human liver cancer tissue, human kidney tissue, mouse testis tissue. |
|---|---|
| Application Notes | WB:1:500 IHC:1:50-1:200 FC:1:50-1:100 IP:1:10-1:20 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
