Application
Fig1: Western blot analysis of Fructose 6 Phosphate Kinase on PC-3M cell lysate using anti-Fructose 6 Phosphate Kinase antibody at 1/500 dilution.Application
Fig2: ICC staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Fructose 6 Phosphate Kinase [JU53-31] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6 Phosphofructokinase Muscle Type antibody 6-phosphofructokinase antibody 6-phosphofructokinase muscle type antibody EC 2.7.1.1 antibody EC 2.7.1.11 antibody GSD7 antibody K6PF_HUMAN antibody MGC8699 antibody muscle type antibody PFK, muscle type antibody PFK-A antibody PFKA antibody PFKL antibody PFKM antibody PFKP antibody PFKX antibody Phosphofructo 1 Kinase Isozyme A antibody Phosphofructo-1-kinase isozyme A antibody Phosphofructokinase 1 antibody Phosphofructokinase M antibody Phosphofructokinase, muscle antibody Phosphofructokinase, muscle type antibody Phosphofructokinase, polypeptide X antibody Phosphofructokinase-M antibody Phosphohexokinase antibody |
| Product description | Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. In human PFK exists as a system of 3 types of subunits, PFKM (muscle), PFKL (liver) and PFKP (platelet) isoenzymes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | 6 Phosphofructokinase Muscle Type antibody
6-phosphofructokinase antibody
6-phosphofructokinase muscle type antibody
EC 2.7.1.1 antibody
EC 2.7.1.11 antibody
GSD7 antibody
K6PF_HUMAN antibody
MGC8699 antibody
muscle type antibody
PFK muscle type antibody PFK-A antibody PFKA antibody PFKL antibody PFKM antibody PFKP antibody PFKX antibody Phosphofructo 1 Kinase Isozyme A antibody Phosphofructo-1-kinase isozyme A antibody Phosphofructokinase 1 antibody Phosphofructokinase M antibody Phosphofructokinase muscle antibody Phosphofructokinase muscle type antibody Phosphofructokinase polypeptide X antibody Phosphofructokinase-M antibody Phosphohexokinase antibody |
|---|---|
| Molecular Weight(MW) | 85 kDa |
| Cellular Localization | Cytoplasm. |
Application
Fig1: Western blot analysis of Fructose 6 Phosphate Kinase on PC-3M cell lysate using anti-Fructose 6 Phosphate Kinase antibody at 1/500 dilution.
Application
Fig2: ICC staining Fructose 6 Phosphate Kinase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Fructose 6 Phosphate Kinase in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Fructose 6 Phosphate Kinase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
Application
Fig7: Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fructose 6 Phosphate Kinase antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of SH-SY-5Y cells with Fructose 6 Phosphate Kinase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | PC-3M, LOVO, SH-SY-5Y, rat brain tissue, human liver cancer tissue, human thyroid gland tissue, mouse skeletal muscle tissue, human kidney tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
