Application
Fig1: ICC staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig2: ICC staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.| Product Name | GABA A Receptor alpha 5 [JB34-19] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | GAA 5 antibody GAA5 antibody GABA(A) receptor subunit alpha-5 antibody GABRA 5 antibody Gabra5 antibody Gamma aminobutyric acid GABA A receptor alpha 5 antibody Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody Gamma-aminobutyric acid receptor subunit alpha-5 antibody GBRA5_HUMAN antibody GC138184 antibody |
| Product description | GAD-65 and GAD-67, glutamate decarboxylases, function to catalyze the production of GABA (gamma-aminobutyric acid). In the central nervous system GABA functions as the main inhibitory transmitter by increasing a Cl- conductance that inhibits neuronal firing. GABA has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. Both GABAA and GABAC are ligand-gated ion channels, however, they are structurally and functionally distinct. Members of the GABAA receptor family include GABAA Rα1-6, GABAA R β1-3, GABAA Rγ1-3, GABAA Rδ, GABAA Rε, GABAA Rρ1 and GABAA Rρ2. The GABAB family is composed of GABAB R1α and GABAB R1β. GABA transporters have also been identified and include GABA T-1, GABA T-2 and GABA T-3 (also designated GAT-1, -2, and -3). The GABA transporters function to terminate GABA action. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHC |
| ICC/IF:1:50-1:200 IHC:1:50-1:200 WB:1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | GAA 5 antibody GAA5 antibody GABA(A) receptor subunit alpha-5 antibody GABRA 5 antibody Gabra5 antibody Gamma aminobutyric acid GABA A receptor alpha 5 antibody Gamma aminobutyric acid GABA A receptor alpha 5 precursor antibody Gamma aminobutyric acid receptor alpha 5 subunit precursor GABA A receptor antibody Gamma-aminobutyric acid receptor subunit alpha-5 antibody GBRA5_HUMAN antibody GC138184 antibody |
|---|---|
| Molecular Weight(MW) | 52 kDa |
| Cellular Localization | Plasma membrane. |
Application
Fig1: ICC staining GABA A Receptor alpha 5 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig2: ICC staining GABA A Receptor alpha 5 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining GABA A Receptor alpha 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-GABA A Receptor alpha 5 antibody. Counter stained with hematoxylin.| Positive Control | 293T, A549, SH-SY-5Y, rat brain tissue, mouse brain tissue. |
|---|---|
| Application Notes | ICC/IF:1:50-1:200 IHC:1:50-1:200 WB:1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
