Application
Fig1: Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.Application
Fig2: ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.Application
Fig7: Flow cytometric analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | GRASP65 [JB36-34] |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FLJ23443 antibody Golgi peripheral membrane protein p65 antibody Golgi phosphoprotein 5 antibody Golgi reassembly and stacking protein 1 antibody Golgi reassembly and stacking protein 65 kDa antibody Golgi reassembly and stacking protein, 65-kD antibody Golgi reassembly stacking protein 1 65kDa antibody Golgi reassembly stacking protein 1 antibody Golgi reassembly stacking protein of 65 kDa antibody Golgi reassembly-stacking protein 1 antibody Golgi reassembly-stacking protein of 65 kDa antibody GOLPH 5 antibody GOLPH5 antibody Gorasp 1 antibody GORASP1 antibody GORS1_HUMAN antibody GRASP 65 antibody GRASP65 antibody MGC118894 antibody MGC118897 antibody P65 antibody |
| Product description | Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis (By similarity). Also mediates, via its interaction with GOLGA2/GM130, the docking of transport vesicles with the Golgi membranes. |
| Immunogen | Recombinant protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Recombinant rabbit |
| Tested Applications | WBICCIFIHCFCIP |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | FLJ23443 antibody
Golgi peripheral membrane protein p65 antibody
Golgi phosphoprotein 5 antibody
Golgi reassembly and stacking protein 1 antibody
Golgi reassembly and stacking protein 65 kDa antibody
Golgi reassembly and stacking protein 65-kD antibody Golgi reassembly stacking protein 1 65kDa antibody Golgi reassembly stacking protein 1 antibody Golgi reassembly stacking protein of 65 kDa antibody Golgi reassembly-stacking protein 1 antibody Golgi reassembly-stacking protein of 65 kDa antibody GOLPH 5 antibody GOLPH5 antibody Gorasp 1 antibody GORASP1 antibody GORS1_HUMAN antibody GRASP 65 antibody GRASP65 antibody MGC118894 antibody MGC118897 antibody P65 antibody |
|---|---|
| Molecular Weight(MW) | 46 kDa (Predicted band size) |
| Cellular Localization | Golgi apparatus. |
| SwissProt ID | Q9BQQ3 |
|---|
Application
Fig1: Western blot analysis of NRCAM on SH-SY5Y cell lysate using anti-NRCAM antibody at 1/1,000 dilution.
Application
Fig2: ICC staining NRCAM in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining NRCAM in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining NRCAM in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-NRCAM antibody. Counter stained with hematoxylin.
Application
Fig7: Flow cytometric analysis of MCF-7 cells with GRASP65 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | THP-1,SKBR3, HUVEC, HepG2, MCF-7, human colon cancer tissue, human uterine tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
