Application
Fig1: Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution. Positive control： Lane1: A549 Lane2: SiHaApplication
Fig2: ICC staining Nfic in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining Nfic in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining Nfic in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Nfic antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.Application
Fig6: Flow cytometric analysis of SiHa cells with Nfic antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | Nfic |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1110019L22Rik antibody 1500041O16Rik antibody AA589446 antibody AI746521 antibody CAAT box transcription factor antibody CCAAT binding transcription factor antibody CCAAT box binding transcription factor antibody CCAAT-box-binding transcription factor antibody CNFI C antibody CTF antibody CTF5 antibody MGC137374 antibody MGC20153 antibody NF I antibody NF I/C antibody NF-I/C antibody NF1 C antibody NF1-C antibody NF1C antibody NFI antibody NFI-C antibody NFI/C antibody NFIC antibody NFIC_HUMAN antibody Nuclear factor 1 antibody Nuclear factor 1 C type antibody Nuclear factor 1 C-type antibody Nuclear factor 1/C antibody Nuclear factor I/C (CCAAT binding transcription factor) antibody Nuclear factor I/C antibody TGGCA binding protein antibody TGGCA-binding protein antibody Transcription factor NFIC antibody |
| Product description | Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. |
| Immunogen | Synthetic peptide within human Nfic 400-450 aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | 1110019L22Rik antibody 1500041O16Rik antibody AA589446 antibody AI746521 antibody CAAT box transcription factor antibody CCAAT binding transcription factor antibody CCAAT box binding transcription factor antibody CCAAT-box-binding transcription factor antibody CNFI C antibody CTF antibody CTF5 antibody MGC137374 antibody MGC20153 antibody NF I antibody NF I/C antibody NF-I/C antibody NF1 C antibody NF1-C antibody NF1C antibody NFI antibody NFI-C antibody NFI/C antibody NFIC antibody NFIC_HUMAN antibody Nuclear factor 1 antibody Nuclear factor 1 C type antibody Nuclear factor 1 C-type antibody Nuclear factor 1/C antibody Nuclear factor I/C (CCAAT binding transcription factor) antibody Nuclear factor I/C antibody TGGCA binding protein antibody TGGCA-binding protein antibody Transcription factor NFIC antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | P08651 |
|---|
Application
Fig1: Western blot analysis of Nfic on different cell lysate using anti-Nfic antibody at 1/500 dilution. Positive control： Lane1: A549 Lane2: SiHa
Application
Fig2: ICC staining Nfic in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining Nfic in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining Nfic in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Nfic antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Application
Fig6: Flow cytometric analysis of SiHa cells with Nfic antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, SiHa, A431. |
|---|---|
| Application Notes | WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
