Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | CD40 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI326936 antibody B cell associated molecule CD40 antibody B cell surface antigen CD40 antibody B cell-associated molecule antibody B-cell surface antigen CD40 antibody Bp50 antibody CD 40 antibody CD40 antibody CD40 antigen (TNF receptor superfamily member 5) antibody CD40 antigen antibody CD40 molecule antibody CD40 molecule, TNF receptor superfamily member 5 antibody CD40 protein antibody CD40 type II isoform antibody CD40L receptor antibody CDw40 antibody GP39 antibody HIGM1 antibody IGM antibody IMD3 antibody MGC9013 antibody Nerve growth factor receptor related B lymphocyte activation molecule antibody OTTHUMP00000031699 antibody OTTHUMP00000031700 antibody p50 antibody T-BAM antibody TBAM antibody TNF receptor superfamily member 5 antibody TNFRSF5 antibody TNR5_HUMAN antibody TRAP antibody Tumor necrosis factor receptor superfamily , member 5 antibody Tumor necrosis factor receptor superfamily member 5 antibody Tumor necrosis factor receptor superfamily member 5 precursor antibody Tumor necrosis factor receptor superfamily, member 5, isoform CRA_a antibody |
| Product description | Resting B cells can be activated and clonally expanded into antibody-producing cells in response to a combination of cell contact and soluble signals provided by primed helper T (Th) cells. A receptor ligand pair central to the trans-mission of this signal is CD40, expressed on the surface of B cells, together with CD40L, expressed on activated T cells. In the presence of such stimulus, IL-4 and IL-13 are capable of triggering immunoglobulin class switching and secretion of IgE. B cells are sensitive to these cytokines only subsequent to CD40/CD40L-driven DNA synthesis. A downstream mediator of the CD40 signaling pathway, designated CRAF, is a member of an expanding family of proteins that contain a conserved cysteine- and histidine-rich RING finger motif. |
| Immunogen | Recombinant protein within human CD40 aa 10-250. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBICCIHCFC |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50 -1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Alternative Names | AI326936 antibody
B cell associated molecule CD40 antibody
B cell surface antigen CD40 antibody
B cell-associated molecule antibody
B-cell surface antigen CD40 antibody
Bp50 antibody
CD 40 antibody
CD40 antibody
CD40 antigen (TNF receptor superfamily member 5) antibody
CD40 antigen antibody
CD40 molecule antibody
CD40 molecule TNF receptor superfamily member 5 antibody CD40 protein antibody CD40 type II isoform antibody CD40L receptor antibody CDw40 antibody GP39 antibody HIGM1 antibody IGM antibody IMD3 antibody MGC9013 antibody Nerve growth factor receptor related B lymphocyte activation molecule antibody OTTHUMP00000031699 antibody OTTHUMP00000031700 antibody p50 antibody T-BAM antibody TBAM antibody TNF receptor superfamily member 5 antibody TNFRSF5 antibody TNR5_HUMAN antibody TRAP antibody Tumor necrosis factor receptor superfamily member 5 antibody Tumor necrosis factor receptor superfamily member 5 antibody Tumor necrosis factor receptor superfamily member 5 precursor antibody Tumor necrosis factor receptor superfamily member 5 isoform CRA_a antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 31kDa |
| Cellular Localization | Plasma membrane. Secreted. |
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Application
Fig6: Flow cytometric analysis of MG-63 cells with CD40 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | Daudi, MG-63, SiHa, rat spleen tissue, human tonsil tissue, SKOV-3. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50 -1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
