Application
Fig1: Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution. Positive control： Lane1, 3: Anti-HEC1 antibody Lane2, 4: Anti-HEC1 antibody, with immunization Recombinant proteinApplication
Fig2: Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | HEC1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | from Entrez Gene ; antibody HEC antibody Highly expressed in cancer antibody Highly expressed in cancer protein antibody Highly expressed in cancer rich in leucine heptad repeats antibody HsHec1 antibody hsNDC80 antibody Kinetochore associated 2 antibody Kinetochore associated protein 2 antibody Kinetochore protein Hec1 antibody Kinetochore protein NDC80 homolog antibody Kinetochore-associated protein 2 antibody KNTC2 antibody ndc80 antibody NDC80 homolog kinetochore complex component antibody NDC80 kinetochore complex component homolog antibody NDC80, S. cerevisiae, homolog of antibody NDC80_HUMAN antibody Retinoblastoma associated protein HEC antibody Retinoblastoma-associated protein HEC antibody TID3 antibody |
| Product description | Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules. |
| Immunogen | Recombinant protein within human HEC1 300-500 aa. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:500 FC:1:50-1:100 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | from Entrez Gene antibody HEC antibody Highly expressed in cancer antibody Highly expressed in cancer protein antibody Highly expressed in cancer rich in leucine heptad repeats antibody HsHec1 antibody hsNDC80 antibody Kinetochore associated 2 antibody Kinetochore associated protein 2 antibody Kinetochore protein Hec1 antibody Kinetochore protein NDC80 homolog antibody Kinetochore-associated protein 2 antibody KNTC2 antibody ndc80 antibody NDC80 homolog kinetochore complex component antibody NDC80 kinetochore complex component homolog antibody NDC80 S. cerevisiae homolog of antibody NDC80_HUMAN antibody Retinoblastoma associated protein HEC antibody Retinoblastoma-associated protein HEC antibody TID3 antibody |
|---|---|
| Molecular Weight(MW) | 74 kDa |
| Cellular Localization | Nucleus. |
| SwissProt ID | O14777 |
|---|
Application
Fig1: Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution. Positive control： Lane1, 3: Anti-HEC1 antibody Lane2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
Application
Fig2: Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | SiHa, HL-60, MG-63. |
|---|---|
| Application Notes | WB:1:500 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
