Application
Fig1:Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution. Positive control： Lane1: A549 Lane3: mouse spleen tissue Lane2: HL-60 Lane4: mouse brain tissueApplication
Fig2: ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig3: ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig4: ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.Application
Fig7:Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.Application
Fig9: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.Application
Fig10: Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Product Name | MCU Rabbit Polyclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex. Activity is regulated by MICU1 and MICU2. At low Ca2+ levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca2+ levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. |
| Immunogen | Recombinant protein within human MCU aa 50-250. |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | FCICCIHCWB |
| WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50 -1:200 FC:1:50-1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ |
| Alternative Names | C109A_HUMAN antibody C10orf42 antibody Calcium uniporter protein mitochondrial antibody Ccdc109a antibody Coiled-coil domain-containing protein 109A antibody HsMCU antibody Mitochondrial Calcium Uniporter antibody |
|---|---|
| Molecular Weight(MW) | Predicted band size 40 kDa |
| Cellular Localization | Mitochondrion. |
Application
Fig1:Western blot analysis of MCU on different lysates using anti-MCU antibody at 1/500 dilution. Positive control： Lane1: A549 Lane3: mouse spleen tissue Lane2: HL-60 Lane4: mouse brain tissue
Application
Fig2: ICC staining MCU in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig3: ICC staining MCU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig4: ICC staining MCU in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Application
Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-MCU antibody. Counter stained with hematoxylin.
Application
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-MCU antibody. Counter stained with hematoxylin.
Application
Fig7:Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MCU antibody. Counter stained with hematoxylin.
Application
Fig8: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-MCU antibody. Counter stained with hematoxylin.
Application
Fig9: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-MCU antibody. Counter stained with hematoxylin.
Application
Fig10: Flow cytometric analysis of A549 cells with MCU antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.| Positive Control | A549, HL-60, mouse spleen tissue lysates, mouse brain tissue lysates, A431, HT-29, rat cerebellum tissue, human lung cancer tissue, mouse brain tissue, human colon tissue, human esophagus tissue. |
|---|---|
| Application Notes | WB:1:500-1:1,000 ICC:1:50-1:200 IHC:1:50 -1:200 FC:1:50-1:100 |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide. |
